Table 3 Bacterial strains and plasmids used in this study

Bacterial strains

Escherichia coli

DH5α

F- φ80dlacZ∆M15 ∆(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rK-mK+) phoA supE44 λ- thi-1 gyrA96 relA1

[52]

HB101

F- mcrB mrr hsdS20(rB- mB-) recA13 leuB6 ara-14 proA2 lacY1 galK2 xyl-5 mtl-1 rpsL20(Sm^R) glnV44 λ-

[53]

CC118λpir

Δ(are-leu) araD ΔlacX74 galE galK phoA20 thi-1 rpsE rpoB argE(Am) recA1 λpir

[54]

S17-1λpir

F’ recA hsdR RP4-2 (Tc::Mu) (Km::Tn7) λpir

[55]

EPI300-T1R

F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15

ΔlacX74 recA1 endA1 araD139 Δ(ara-leu)7697

galU galK λ- rpsL nupG trfA tonA dhfr

Epicentre

Klebsiella pneumoniae

C3091

Clinical urinary tract infection isolate; St^R

[56]

C3091∆fim::tet∆mrk::kan

C3091 with fim and mrk clusters deleted; St^R, Tet^R, Kan^R

[23]

KR116

Clinical blood stream infection isolate

This work

KR116 ∆fim2K::kan

KR116 with an insertion-deletion mutation in fim2K where 125 bp has been replaced by a kanamycin resistance cassette; Kan^R

This work

KR2107

Spontaneous streptomycin resistant derivative of KR116; St^R

This work

KR2107∆fim

KR2107 with fim deleted; St^R, Tet^R

This work

KR2107∆fim2

KR2107 with fim2 deleted; St^R, Kan^R

This work

KR2107∆fim∆fim2

KR2107 with fim and fim2 deleted; St^R, Tet^R, Kan^R

This work

Plasmids

pDS132

Lambda pir-based suicide vector; Cml^R

[57]

pBluescript II KS+

High copy number cloning vector; Amp^R

Fermentas

pWSK129

Low copy number cloning vector; Kan^R

[58]

pKOBEG-Apra

Lambda Red expression plasmid, P_BAD promoter; Apr^R

[59]

pRT733

Source of Kan^R cassette; Kan^R

[60]

pCC2FOS

Fosmid cloning vector; Cml^R

Epicentre

pTRC99a

IPTG inducible expression vector with P_TRC promoter and lacI^q; Amp^R

[61]

pJTOOL-7

Derivate of pTRC99a with an added NotI cut site in multiple the cloning site; Amp^R

This work

pJKO-4a

pDS132 with SOE-PCR fragment for tagging of fim2K (fim2K::Kan); Cml^R, Kan^R

This work

pJFos-1

40.6 kb fim2K::Kan-containing fragment cloned into pCC2FOS; Cml^R, Kan^R

This work

pJFos-4

26.1 kb fim2K::Kan-containing fragment cloned into pCC2FOS; Cml^R, Kan^R

This work

pFim2-HCN

9.4 kb PCR fragment containing the fim2 locus cloned into the NotI site of pBluescript II KS+; Amp^R

This work

pFim2-LCN

9.4 kb PCR fragment containing the fim2 locus cloned into the NotI site of pWSK129; Amp^R

This work

pFim2-Ptrc

9.0 kb PCR fragment containing the fim2 locus cloned into the NotI/SbfI site of pJTOOL-7. IPTG inducible; Amp^R

This work