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Table 3 Bacterial strains and plasmids used in this study

From: Characterization of a novel chaperone/usher fimbrial operon present on KpGI-5, a methionine tRNA gene-associated genomic island in Klebsiella pneumoniae

Bacterial strain or plasmid Relevant characteristicsa Reference or Source
Bacterial strains   
Escherichia coli   
DH5α F- φ80dlacZ∆M15 ∆(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rK-mK+) phoA supE44 λ- thi-1 gyrA96 relA1 [52]
HB101 F- mcrB mrr hsdS20(rB- mB-) recA13 leuB6 ara-14 proA2 lacY1 galK2 xyl-5 mtl-1 rpsL20(SmR) glnV44 λ- [53]
CC118λpir Δ(are-leu) araD ΔlacX74 galE galK phoA20 thi-1 rpsE rpoB argE(Am) recA1 λpir [54]
S17-1λpir F’ recA hsdR RP4-2 (Tc::Mu) (Km::Tn7) λpir [55]
EPI300-T1R F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15
ΔlacX74 recA1 endA1 araD139 Δ(ara-leu)7697
galU galK λ- rpsL nupG trfA tonA dhfr
Epicentre
Klebsiella pneumoniae   
C3091 Clinical urinary tract infection isolate; StR [56]
C3091∆fim::tet∆mrk::kan C3091 with fim and mrk clusters deleted; StR, TetR, KanR [23]
KR116 Clinical blood stream infection isolate This work
KR116 ∆fim2K::kan KR116 with an insertion-deletion mutation in fim2K where 125 bp has been replaced by a kanamycin resistance cassette; KanR This work
KR2107 Spontaneous streptomycin resistant derivative of KR116; StR This work
KR2107∆fim KR2107 with fim deleted; StR, TetR This work
KR2107∆fim2 KR2107 with fim2 deleted; StR, KanR This work
KR2107∆fimfim2 KR2107 with fim and fim2 deleted; StR, TetR, KanR This work
Plasmids   
pDS132 Lambda pir-based suicide vector; CmlR [57]
pBluescript II KS+ High copy number cloning vector; AmpR Fermentas
pWSK129 Low copy number cloning vector; KanR [58]
pKOBEG-Apra Lambda Red expression plasmid, PBAD promoter; AprR [59]
pRT733 Source of KanR cassette; KanR [60]
pCC2FOS Fosmid cloning vector; CmlR Epicentre
pTRC99a IPTG inducible expression vector with PTRC promoter and lacIq; AmpR [61]
pJTOOL-7 Derivate of pTRC99a with an added NotI cut site in multiple the cloning site; AmpR This work
pJKO-4a pDS132 with SOE-PCR fragment for tagging of fim2K (fim2K::Kan); CmlR, KanR This work
pJFos-1 40.6 kb fim2K::Kan-containing fragment cloned into pCC2FOS; CmlR, KanR This work
pJFos-4 26.1 kb fim2K::Kan-containing fragment cloned into pCC2FOS; CmlR, KanR This work
pFim2-HCN 9.4 kb PCR fragment containing the fim2 locus cloned into the NotI site of pBluescript II KS+; AmpR This work
pFim2-LCN 9.4 kb PCR fragment containing the fim2 locus cloned into the NotI site of pWSK129; AmpR This work
pFim2-Ptrc 9.0 kb PCR fragment containing the fim2 locus cloned into the NotI/SbfI site of pJTOOL-7. IPTG inducible; AmpR This work
  1. a Amp ampicillin; Apr apramycin; Cml chloramphenicol; Kan kanamycin; St streptomycin; Tet tetracycline. Superscript R indicates resistance.