Features of the novel KpGI-5 genomic island in K. pneumoniae KR116. (A) Genetic organisation of KpGI-5 shown lying between the species-conserved upstream flank (UF) and downstream flank (DF) sequences. The eight putative fimbrial genes are labelled fim2A–fim2K. Closest BLASTP similarities for these and other predicted KpGI-5-encoded proteins are described in Table 1. KpGI-5 segments indicated by double arrows map to G + C % transitions as indicated by G + C profile. The thin horizontal lines on the G + C % graph represent the average G + C content of the K. pneumoniae MGH78578 genome (57.4%) and the entire KpGI-5 island (44.0%). The 20.8% and 65.0% G + C content lines correspond to the minimum and maximum G + C % calculated over an 80 bp window, respectively. (B) Alignment of the tRNA-proximal (DRP) and tRNA-distal (DRD) 46 bp direct repeat (DR) sequences associated with KpGI-5. DRP comprises the 3’ end of met56.