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Table 2 PCR primers used in this study

From: Re-evaluation of the significance of penicillin binding protein 3 in the susceptibility of Listeria monocytogenes to β-lactam antibiotics

Primer

Sequence (5'→ 3')

HlyAa

GCGGGTACCAGGTAGAGCGGACATCCATTG

HlyBb, c, d

GTTTTA GGATCC CCCGGGGGGTTTCACTCTCCTTCTAC

HlyCb, c

CCCGGG GGATCCTAAAACCGCTTAACACACACG

HlyDe

GCGTCTAGATTCTTCCCCGACAGAATCTGC

NisR F

CCCACTAAACAATCGGAGG

NisK Rc

GCGGGATCCCAGAAATTAAACCAAACAAAATTTTC

Oepbp3 F

CGTGAAACTAAATTTTAGAAAAAAGAAAAAAG

Oepbp3 Rf

GCGGCATGCGATTAATTTTCGGTTTGTTCTGATTG

  1. a Nucleotide substitutions to create KpnI site are underlined
  2. b Nucleotide substitutions to create SmaI site are underlined
  3. c Nucleotide substitutions to create BamHI site are in boldface
  4. d Overhang complementary to SOE primer is in italics
  5. e Nucleotide substitutions to create XbaI site are underlined
  6. f Nucleotide substitutions to create SphI site are underlined
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BMC Microbiology

ISSN: 1471-2180

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