Table 2 Results from numerical coverage analysis performed by comparing primer and probe sequences from BactQuant and the published qPCR assays against >670,000 16 S rRNA gene sequences from RDP
From: BactQuant: An enhanced broad-coverage bacterial quantitative real-time PCR assay
BactQuant | Published qPCR Assay | Coverage Improvement | |
|---|---|---|---|
A. Perfect match using full length primers and probe | |||
Phyla | 91.2% (31/34) | 61.8% (21/34) | + 29.4% |
Genus | 96.2% (1778/1849) | 80.3% (1485/1849) | +15.8% |
Species* | 83.5% (74725/89537) | 66.3% (59459/89646) | +17.2% |
All Sequences* | 78.0% (524118/671595) | 60.9% (409584/672060) | +17.1% |
B. Perfect match using 8-nt primers with full length probe | |||
Phyla | 91.2% (31/34) | 67.7% (23/34) | +23.5% |
Genus | 97.7% (1806/1849) | 82.1% (1518/1849) | +15.6% |
Species* | 89.1% (79759/89537) | 70.9% (63533/89646) | +18.2% |
All Sequences* | 84.4% (566685/671595) | 65.6% (441017/672060) | +18.8% |