Table 1 Primer and probe sequences of BactQuant, the new 16 S rRNA gene-based quantitative real-time PCR (bold letters denotes degenerate base)
From: BactQuant: An enhanced broad-coverage bacterial quantitative real-time PCR assay
BactQuant | Tm (°C) | E. coli region | |
|---|---|---|---|
Forward Primer | 5′- CCTACGGGDGGC WGCA-3′ | 55.9–58.4 | 341–356 |
Reverse Primer | 5′- GGACTACHVGGGT MTCTAATC -3′ | 57.5–63.3 | 786–806 |
Probe | (6FAM) 5′-CAGCAGCCGCGGTA-3′ (MGBNFQ) | 68.0 | 519–532 |