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Table 2 Positions and putative functions of open reading frames identified in plasmid N3

From: Fitness of Escherichia coli strains carrying expressed and partially silent IncN and IncP1 plasmids

Gene/orf Position Putative Function Closest match (Accession)1 Protein identity (%)
repA 3-722 Initiation of plasmid replication pKOX105 (ADH29527) 100
ardK 1282-1623 Regulation of ccg genes pEC_L46 (ADL14210) 100
mpr 1638-2429 Zinc metalloproteinase pLEW517 (YP_001096387) 100
mucB 2580-3845 UV protection pKOX105 100
mucA 3833-4273 UV protection pEC_L46 100
ardB 4688-5113 Type I antirestriction system R46 (NP_511215) 99
ardR 5171-5575 Regulator of CUP controlled ccgEII regulon pEC_L46 100
ccgEIII 5585-5824 Unknown R46 100
ccgAI 7332-7511 Regulation of ccgAII expression R46 100
ccgAII 7566-7886 Prevention of RecA overproduction pKOX105 100
pN3_011 7997-8341 Unknown R46 100
stbC 8523-8891 Stable plasmid inheritance R46 100
stbB 8893-9609 Stable plasmid inheritance pKOX105 100
stbA 9618-10037 Stable plasmid inheritance pKOX105 100
traK 10528-10944 Conjugal transfer protein pKOX105 100
traJ 10946-12475 Conjugal transfer protein pKP96 (YP_002332894) 100
traI 12475-15717 Conjugal nickase and helicase pKP96 100
fipA 15717-16343 Fertility inhibition of IncP plasmids pKM101 (AAC63100) 100
nuc 16517-17050 Endonuclease pKOX105 100
traG 17050-18045 Conjugal transfer protein pKOX105 100
traF 18087-19247 Conjugal transfer protein pKOX105 100
traO 19247-20131 Conjugal transfer protein pEC_L46 100
traE 20142-20840 Conjugal transfer protein pEC_L46 100
traN 20830-20967 Conjugal transfer protein pEC_L46 100
traD 21059-22099 Conjugal transfer protein pEC_L46 100
eex 22115-22342 Entry exclusion pKOX105 100
traC 22350-23063 Conjugal transfer protein pKOX105 100
traB 23081-25681 Conjugal transfer protein pKOX105 100
traA 25681-25998 Conjugal transfer protein pKOX105 100
traM 26048-26341 Conjugal transfer protein pKOX105 100
korA 26351-26632 Unknown R46 100
traL 26641-27375 Conjugal transfer protein pKOX105 100
korB 27484-27789 DNA binding protein pKOX105 99
pN3_034 27805-28149 Unknown pKOX105 100
kikA 28146-28460 Killer protein of TrbM family pKOX105 100
pN3_036 28496-28807 Unknown R46 100
mrr 28863-29504 Restriction endonuclease pKOX105 100
pN3_038 29509-29715 Unknown pKP96 100
EcoRII met 30055-31530 Modification methylase pKOX105 100
EcoRII 31564-32778 Type-2 restriction enzyme pKOX105 100
tnpA 33039-33833 IS6100 transposase pEK499 (YP_003108355) 100
pN3_042 33999-34724 Unknown pAPEC-O1-R (YP_001481449) 100
sul1 34938-35777 Sulphonamide resistant dihydropteroate synthase R46 100
qacEΔ1 35771-36118 Quaternary ammonium compound resistance, truncated R46 100
aadA2 36282-37073 Aminoglycoside adenyltransferase p1206 (ACC77487) 100
intI1 37219-38232 Class 1 integrase pKOX105 100
uvp1 38625-39194 Site specific recombinase pKOX105 100
tnpA 39506-40210 IS26 transposase pKOX105 100
pN3_049 40247-40750 Putative shikimate dehydrogenase (repeat protein) Pantotea sp. (YP_004116848) 59
tet(A) 41265-42464 Tetracycline efflux protein pQKp331H (ABS19074) 100
tetR 42592-43233 Repressor protein for Tet(A) pQKp331H 100
pN3_052 43438-43941 Unknown No good match  
pN3_053 44147-44563 Unknown pLVPK (NP_943518) 59
tnp orfA 44921-45265 IS911 transposase, truncated Shigella flexneri 2a str. 2457 T (NP_835957) 80
pN3_055 45468-46295 Putative bacitracin resistance protein Acinetobacter sp. DR1 (YP_003733303) 62
pN3_056 46450-47589 Putative amino acid racemase Pectobacterium carotovorum PC1 (YP_003017826) 73
pN3_057 47686-48597 Putative LysR-type regulator Shewanella halifaxensis HAW-EB4 (YP_001674862) 56
pN3_058 48594-49526 Putative amino acid dehydrogenase/cyclodeaminase Pectobacterium carotovorum subsp. brasiliensis PBR1692
(ZP_03825565)
72
pN3_059 50018-50623 Putative sodium:dicarboxylate symporter Burkholderia dolosa AUO158 (ZP_04944635) 56
tnpA 50681-51385 IS26 transposase pKOX105 100
hsdM 51636-53192 Type I restriction enzyme EcoprrI M protein Escherichia coli B185 (ZP_06660389) 90
pN3_062 53656-54165 Unknown pKOX105 90
  1. 1 Where more than one protein shares the exact same identity with pN3 an example is given