Analysis of the LIC11834 and LIC12253 genes and their transcripts among different leptospiral strains. (A) Analysis by PCR of the LIC11834 (2) and LIC12253 (3) genes in pathogenic serovars (L. interrogans, L.borgpetersenii, L. kirshnery, L. noguchi and L. santarosai) and in the non - pathogenic L. biflexa strain. 16 S rRNA gene expression was used as an internal control (1). The negative control contained no DNA, indicated by (−). (B) Analysis of the LIC11834 (2) and LIC12253 (3) transcripts by RT - PCR using 2 μg total RNA extracted from different serovars belonging to the pathogenic species and the saprophytic L. biflexa serovar Patoc strain Patoc. 16 S rRNA gene expression was used as an internal control (1). Reverse transcriptase present, +; reverse transcriptase omitted, -. The negative control contained no cDNA, indicated by (−).