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Table 2 C.elegans single gene mutants used in this study

From: Control of intestinal bacterial proliferation in regulation of lifespan in Caenorhabditis elegans

Strain Genotype Function Relevant C. elegans phenotype Reference*
N2 Wild type   Reference C. elegans strain [20]
daf-2 (e1370)III Insulin-like receptor gene Extended lifespan, increased resistance to heat, oxidative stress, and pathogens. [14, 22]
age-1 (hx546)II Phosphatidylinositol-3 kinase. Downstream of daf-2. Similar to daf-2 [22, 83]
daf-16 (mu86)I Fork-head transcription factor. Negatively regulated by the daf-2 pathway. Decreased lifespan, decreased resistance to heat, oxidative stress, and pathogens. [22, 84]
lys-7 (ok1384)V Lysozyme Induced by S. marcescens infection [31]
spp-1 (ok2703) Saposin-like protein Active against E. coli and expressed in the intestine [85]
sod-3 (gk235)X Superoxide dismutase Increased susceptibility to E. faecalis [42]
ctl-2 (ok1137)II Catalase Decreased lifespan, increased susceptibility to E. faecalis [42, 44]
dbl-1 (nk3)V Homologue of mammalian TGF-β Enhanced susceptibility to pathogens [31, 86]
lys-1 (ok2445) Lysozyme Induced by S. marcescens infection [31]
pmk-1 (km25) p38 MAP kinase homolog Enhanced susceptibility to pathogens [27]
tol-1 (nr2033)I Sole Tol-like receptor. Unable to avoid pathogenic bacteria. Susceptible to killing by gram negative bacteria. . [35, 36]
trx-1 (ok1449)II Thioredoxin Decreased lifespan [47, 48]
phm-2 (ad597)I Pharynx morphogenesis Defective terminal bulb. Allows greater numbers of intact bacteria to enter the intestinal tract. [54]
clk-1 (e2519)III Coenzyme Q Mitochondrial function Extended lifespan [56]
  1. * All strains were provided by the Caenorhabditis Genetic Center, University of Minnesota