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Table 1 Lifespan and intestinal colonization of C.elegans N2 and mutants with growth on E. coli or Salmonella lawnsa

From: Control of intestinal bacterial proliferation in regulation of lifespan in Caenorhabditis elegans

   E. coli OP50 S. typhimurium SL1344
Genotype Symbol TD50
(Mean ± SD)
Day 2 log10 intestinal cfu (Mean ± SD) TD50
(Mean ± SD)
Day 2 log10 intestinal cfu (Mean ± SD)
N2 12.93 ± 0.50 2.76 ± 0.22 10.87 ± 1.37 3.22 ± 0.07
daf-2 26.45 ± 1.34^^ 1.70 ± 0.12^^ 20.17 ± 0.29^^ 1.87 ± 0.15^^
age-1 18.75 ± 0.35^^ 2.48 ± 0.32 13.70 ± 0.14^ 2.36 ± 0.48^
daf-16 8.05 ± 0.38^^ 3.30 ± 0.19 5.53 ± 0.23^^ 3.55 ± 0.15^
lys-7 9.30 ± 0.74^ 2.93 ± 0.39 8.83 ± 0.25^ 3.31 ± 0.28
spp-1 9.80 ± 0.59^ 2.67 ± 0.27 8.70 ± 0.14^ 3.41 ± 0.23
sod-3 11.90 ± 1.01 2.87 ± 0.24 10.93 ± 1.23 3.45 ± 0.25
ctl-2 9.48 ± 0.29^ 2.69 ± 0.18 8.98 ± 0.67^ 3.88 ± 0.14^
dbl-1 5.80 ± 0.57^^ 3.35 ± 0.06 4.75 ± 0.79^^ 3.86 ± 0.19^
lys-1 10.00 ± 0.40^ 2.60 ± 0.22 8.95 ± 0.44^ 3.12 ± 0.24
pmk-1 7.40 ± 0.16^^ 2.58 ± 0.34 6.10 ± 0.99^^ 3.71 ± 0.78^
tol-1 10.53 ± 0.31^^ 2.81 ± 0.15 8.98 ± 0.79^ 3.53 ± 0.18^
trx-1 7.70 ± 0.14^^ 2.95 ± 0.17 6.83 ± 0.38^^ 3.30 ± 0.38
  1. a Worms were age-synchronized by a bleaching procedure. Embryos were placed on mNGM agar plates containing E. coli OP50 or S. typhimurium SL1344 and incubated at 25°C. The L4 stage was designated as day 0. A total of 100 worms were used per lifespan assay. Bacterial colonization of the intestinal tract was determined at day 2 by washing and grinding 10 worms, and plating worm lysates on MacConkey agar. All assays were performed at least three times
  2. ^p< 0.05, compared to N2
  3. ^^p< 0.001, compared to N2