Density of bacterial accumulation in the C. elegans intestine by worm age and genotype, and bacterial strain. Survival of N2 C. elegans and DAF-2 pathway mutants when grown on lawns of E. coli OP50 (Panel A) or S. typhimurium SL1344 (Panel B). Intestinal density of viable E. coli OP50 (Panel C) or S. typhimurium SL1344 (Panel D) in N2 C. elegans and DAF-2 pathway mutants. Panel E: Intestinal load of E. coli OP50 (dark bars) or S. typhimurium SL1344 (grey bars) within N2 C. elegans and DAF-2 pathway mutants on day 2 (L4 stage + 2 days) of their lifespan. Data represent Mean ± SD from experiments involving 30 worms/group. Significant difference (p < 0.05) compared to N2 worms exposed to E. coli OP50 or S. typhimurium SL1344, indicated by * or **, respectively.