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Table 2 Results of rifampicin susceptibility testing and rpoB genotyping

From: Genetic basis of rifampicin resistance in methicillin-resistant Staphylococcus aureus suggests clonal expansion in hospitals in Cape Town, South Africa

Clonal type1 (clonal complex) PFGE cluster2 (n)/spa type (n) Isolate origin (isolate name) Rifampicin MIC (mg/L)3 Amino acid position4 Nucleotide substitution Amino acid substitution
ST22- MRSA-IV (22) Sporadic isolate (1)/t032 (1) Cape Town, RSA5 ≤ 0.016 498 GCG → GCT -
     554 CAT → CAC -
     599 AAT → AAC -
ST36- MRSA-II (30) F (1)/t021 (1) Cape Town, RSA ≤ 0.016 474 AAC → AAT -
     498 GCG → GCT -
     502 GTA → GTG -
     518 ACA → ACG -
ST5- MRSA-I (5) C (1)/t045 (1) Cape Town, RSA ≥ 256 481 C AT → T AT H481Y
     498 GCG → GCT -
     630 AAT → AAC -
     658 GGT → GGA -
ST612- MRSA-IV (8) D (2), E (5), sporadic isolates (2)/t064 (3), t1443 (5), t1257 (1) Cape Town, RSA ≥ 256 481 C AT → A AT H481N
     498 GCG → GCT -
     512 CGT → CGC -
     527 ATT → ATG I527M
ST612- MRSA-IV (8) ND6 (2)/t064 (2) RSA (N83; N84) ≥ 256 481 C AT → A AT H481N
     498 GCG → GCT -
     512 CGT → CGC -
     527 ATT → ATG I527M
ST612- MRSA-IV (8) ND (1)/t064 (1) Australia (04-17052) ≥ 256 481 C AT → A AT H481N
     498 GCG → GCT -
     512 CGT → CGC -
     527 ATT → ATG I527M
ST612- MRSA-IV (8) ND (1)/t7571 (1) Australia (09-15534) ≥ 256 481 C AT → A AT H481N
     498 GCG → GCT -
     512 CGT → CGC -
     527 ATT→ATG I527M
     579 AA A→AG A K579R
  1. 1 Clonal types are indicated using the current international nomenclature (sequence type (ST) - antimicrobial phenotype - staphylococcal cassette chromosome mec (SCCmec) type)
  2. 2 PFGE, pulsed-field gel electrophoresis
  3. 3 As determined by E-test
  4. 4 S. aureus co-ordinates
  5. 5 RSA, Republic of South Africa
  6. 6 ND, not determined