Table 1 Oligonucleotide primers used in this study
Gene | Forward (5'-3') | Reverse (5'-3') |
|---|---|---|
Cloning | Â | Â |
flaB | GGGAACTTGATTAGCCTGCGCAAT | TCGAGCTTCTGATGATGCTGCT |
rpoS | CTTGCAGGACAAATACAAAGAGGC | TGGGACTATTGTCCAGGTTATATCTTT |
ospC | CTGCTGATGAGTCTGTTAAAGGGC | TTTGGACTTTCTGCCACAACAGGG |
ospA | GCGTTTCAGTAGATTTGCCTGGTG | CCCTCTAATTTGGTGCCATTTGAGTCG |
dbpA | CTTATATCATGTGGACTAACAGGAGC | AGCACTCCTTGAGCTGTAGTTGGA |
qRT-PCR | Â | Â |
flaB | TGATTAGCCTGCGCAATCATT | AATGACAGATGAGGTTGTAGCAGC |
rpoS | CTGGACAAAGAAATAGAGGGATCTG | CAAGGGTAATTTCAGGGTTAAAAGAA |
ospC | GTACTAAAACTAAAGGTGCTGAAGAA | GCATCTCTTTAGCTGCTTTTGACA |
ospA | GGCGTAAAAGCTGACAAAAGTAAAGT | TGTTTTGCCATCTTCTTTGAAAAC |
dbpA | ACGAAGCGCTAAAGACATTACAGA | GGCATCAAAATTTACGCCCTTA |