Identification of genes expressed in cultures of E. coli lysogens carrying the Shiga toxin-encoding prophage Φ24B

Table 4 Bacterial strains, plasmid and phages used in the study

E.colistrains, plasmids and phages	Relevant Genotype	Reference
BL21-AI	F- ompT hsdSB(rB-, mB-) gal dcm (DE3), arabinose inducible T7 RNA polymerase	Invitrogen, Paisley, U.K.
MC1061	F^- Δ(ara-leu)7697 Δ(codB-lacI)3 galK16 λ^- mcrA0 rpsL150(strR) mcrB1	[18]
DM1187	F- dam-13::Tn9(Cm^R) dcm- mcrB hsdR-M + gal1 ara- lac- thr- leu- tsxR	[45]
TOP10	F- mcrA Φ80lacZΔM15 recA⁺	Invitrogen, Paisley, U.K.
pCR ^® -Blunt	lacZ α, Kan^R, ccdB	Invitrogen, Paisley, U.K.
pET30c	Expression vector with T7 promoter, Kan^R, Tet^R,	Novagen, Notts, UK
Φ24 _B	Stx2-phage, ΔstxA₂::aph3	[14]

ISSN: 1471-2180