Skip to main content

Advertisement

Table 4 Bacterial strains, plasmid and phages used in the study

From: Identification of genes expressed in cultures of E. coli lysogens carrying the Shiga toxin-encoding prophage Φ24B

E.colistrains, plasmids and phages Relevant Genotype Reference
BL21-AI F- ompT hsdSB(rB-, mB-) gal dcm (DE3), arabinose inducible T7 RNA polymerase Invitrogen, Paisley, U.K.
MC1061 F- Δ(ara-leu)7697 Δ(codB-lacI)3 galK16 λ- mcrA0 rpsL150(strR) mcrB1 [18]
DM1187 F- dam-13::Tn9(CmR) dcm- mcrB hsdR-M + gal1 ara- lac- thr- leu- tsxR [45]
TOP10 F- mcrA Φ80lacZΔM15 recA+ Invitrogen, Paisley, U.K.
pCR ® -Blunt lacZ α, KanR, ccdB Invitrogen, Paisley, U.K.
pET30c Expression vector with T7 promoter, KanR, TetR, Novagen, Notts, UK
Φ24 B Stx2-phage, ΔstxA2::aph3 [14]