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Table 2 Protein identities according to the MASCOT database

From: Identification of genes expressed in cultures of E. coli lysogens carrying the Shiga toxin-encoding prophage Φ24B

P♯

Gene name

Access No.

pI/MW (Da)

Description

Sequencea Coverage (%)

MASCOTb Score

Peptidesc matches

Estimated pI/MW (Da)

MASCOT Database Identified in

1

P1

 

5.28/33860

Identical to hypothetical protein p78 from 933 Wd

32

63*

6

5.50/40000

1

2

P2

 

5.27/17096

Similar to hypothetical protein p23 from 933 W

42

39

5

5.00/15000

1

3

P3

 

5.09/13472

Similar to hypothetical protein p24 from 933 W

33

55

3

5.6/8000

1

4

P4

 

5.14/25800

Identical to exo of 933 W

29

52

5

5.5/40000

1

5

P5

 

5.29/7336

Not known homologue

83

41

2

7.00/7000

1

6

P6

 

5.22/13628

Identical to hypothetical protein Stx2Ip064e

38

30

4

7.00/10000

1

7

nanA2

Q6KD26

5.77/34077

N-acetylneuraminate lyse 2

21

47

4

5.3/35000

2

8

gadB

CAQ31981

5.29/52634

Glutamate decarboxylase beta

23

57

7

5.3/35000

2

9

sodB

P0AGD5

5.58/21121

Superoxide dismutase [Fe]

40

53

6

4.00/100000

2

10

napA

AAC75266

8.23/92983

Nitrate reductase

14

49

7

5.5/100000

2

11

tig

AAA62791

4.73/47994

Trigger factor

24

58*

7

3.5/6000

2

12

UTI89_C3021

Q1R837

4.70/6971

Hypothetical protein

70

42

2

5.5/7000

2

13

2FPKA

ZP_04873224

5.24/32497

6-phosphofructokinase

23

46

5

5.3/50000

2

14

gcpE

S23058

5.87/40658

1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

16

38

4

5.5/80000

2

15

aceE

P0AFG9

5.46/99475

Pyruvate dehydrogenase E1 component

10

60*

7

5.4/100000

2

16

bfpK

Q9S141

7.63/18294

BfpK

54

49

3

6.4/25000

2

17

ychN

P0AB53

5.02/12685

ychN

46

38

2

5.3/100000

2

18

UTI89_C1147

Q1RDD

5.57/24945

Hypothetical protein

15

38

4

5.7/30000

2

19

ompC

Q9RH85

4.55/40474

Outer membrane protein

18

44

4

5.5/40000

2

20

ECs1247

G90784

4.74/25144

Hypothetical protein

26

39

5

6.5/35000

2

21

UTI89_C2748

Q1R8V6

10.19/10724

Hypothetical protein

44

50

4

6.4/8000

2

22

nirB

E86001

5.79/93112

Nitrate reductase (NAD(P)H) Subunit

10

53

8

5.3/100000

2

23

yagP

CAQ30761

5.65/15401

yagP protein

36

43

3

5.3/10000

2

24

rhsF

Q47284

5.69/23342

RhsF

18

42

4

5.69/8000

2

  1. Table represents matches to E. coli proteins in the MASCOT database and matches to Φ24B proteins in the University of Liverpool local MASCOT database
  2. a percentage of sequence of the matched protein that is covered by the experimental MS.
  3. b logarithm of the probability that the match between the experimental data and a protein sequence in the database is a random event.
  4. c number of peptides that match the protein in the database
  5. d 933 W is an Stx2 phage described by Plunkett et al. [16].
  6. e Stx2 is an Stx2 phage described by Sato et al. [20].
  7. *represents significant matches (p-value < 0.05)
  8. 1 University of Liverpool local MASCOT database; 2 general MASCOT database