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Table 2 Protein identities according to the MASCOT database

From: Identification of genes expressed in cultures of E. coli lysogens carrying the Shiga toxin-encoding prophage Φ24B

P♯ Gene name Access No. pI/MW (Da) Description Sequencea Coverage (%) MASCOTb Score Peptidesc matches Estimated pI/MW (Da) MASCOT Database Identified in
1 P1   5.28/33860 Identical to hypothetical protein p78 from 933 Wd 32 63* 6 5.50/40000 1
2 P2   5.27/17096 Similar to hypothetical protein p23 from 933 W 42 39 5 5.00/15000 1
3 P3   5.09/13472 Similar to hypothetical protein p24 from 933 W 33 55 3 5.6/8000 1
4 P4   5.14/25800 Identical to exo of 933 W 29 52 5 5.5/40000 1
5 P5   5.29/7336 Not known homologue 83 41 2 7.00/7000 1
6 P6   5.22/13628 Identical to hypothetical protein Stx2Ip064e 38 30 4 7.00/10000 1
7 nanA2 Q6KD26 5.77/34077 N-acetylneuraminate lyse 2 21 47 4 5.3/35000 2
8 gadB CAQ31981 5.29/52634 Glutamate decarboxylase beta 23 57 7 5.3/35000 2
9 sodB P0AGD5 5.58/21121 Superoxide dismutase [Fe] 40 53 6 4.00/100000 2
10 napA AAC75266 8.23/92983 Nitrate reductase 14 49 7 5.5/100000 2
11 tig AAA62791 4.73/47994 Trigger factor 24 58* 7 3.5/6000 2
12 UTI89_C3021 Q1R837 4.70/6971 Hypothetical protein 70 42 2 5.5/7000 2
13 2FPKA ZP_04873224 5.24/32497 6-phosphofructokinase 23 46 5 5.3/50000 2
14 gcpE S23058 5.87/40658 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase 16 38 4 5.5/80000 2
15 aceE P0AFG9 5.46/99475 Pyruvate dehydrogenase E1 component 10 60* 7 5.4/100000 2
16 bfpK Q9S141 7.63/18294 BfpK 54 49 3 6.4/25000 2
17 ychN P0AB53 5.02/12685 ychN 46 38 2 5.3/100000 2
18 UTI89_C1147 Q1RDD 5.57/24945 Hypothetical protein 15 38 4 5.7/30000 2
19 ompC Q9RH85 4.55/40474 Outer membrane protein 18 44 4 5.5/40000 2
20 ECs1247 G90784 4.74/25144 Hypothetical protein 26 39 5 6.5/35000 2
21 UTI89_C2748 Q1R8V6 10.19/10724 Hypothetical protein 44 50 4 6.4/8000 2
22 nirB E86001 5.79/93112 Nitrate reductase (NAD(P)H) Subunit 10 53 8 5.3/100000 2
23 yagP CAQ30761 5.65/15401 yagP protein 36 43 3 5.3/10000 2
24 rhsF Q47284 5.69/23342 RhsF 18 42 4 5.69/8000 2
  1. Table represents matches to E. coli proteins in the MASCOT database and matches to Φ24B proteins in the University of Liverpool local MASCOT database
  2. a percentage of sequence of the matched protein that is covered by the experimental MS.
  3. b logarithm of the probability that the match between the experimental data and a protein sequence in the database is a random event.
  4. c number of peptides that match the protein in the database
  5. d 933 W is an Stx2 phage described by Plunkett et al. [16].
  6. e Stx2 is an Stx2 phage described by Sato et al. [20].
  7. *represents significant matches (p-value < 0.05)
  8. 1 University of Liverpool local MASCOT database; 2 general MASCOT database