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Figure 2 | BMC Microbiology

Figure 2

From: Shigella flexneriutilize the spectrin cytoskeleton during invasion and comet tail generation

Figure 2

Spectrin cytoskeletal proteins are recruited to internalized S. flexneri. Cells were infected for 2.5 hours prior to fixation and treatment with antibodies targeted to spectrin, adducin or p4.1, together with probes for F-actin and DAPI (to visualize the DNA within the bacteria). a) All three proteins are recruited to the regions containing the internalized bacteria (arrowheads). Spectrin and adducin panels show instances where spectrin cytoskeletal proteins were concentrated in the absence of actin. Scale bars are 5 μm. b) Quantification of spectrin, p4.1 and adducin recruitment to internalized bacteria. 200 internalized bacteria were counted, in three separate experiments, to observe if they had recruited spectrin cyskeletal proteins to the bacteria. * p < 0.05

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