From: Role of the gerA operon in L-alanine germination of Bacillus licheniformisspores
strain or plasmid | description, phenotype or genotype relevant for this studya | reference |
---|---|---|
Strains | Â | Â |
Escherichia coli TOP10 | One Shot® TOP10 electro/chemically competent E. coli for cloning | Invitrogen |
MW3 | Bacillus licheniformis DSM13 (ΔhsdR1, ΔhsdR2) | [50] |
NVH-1307 | B. licheniformis MW3ΔgerAA::spc. SpR. | This study |
NVH-1311 | NVH-1307 with pHT315_MW3gerA. SpR and EmR. | This study |
ATCC 14579 | Bacillus cereus type strain | |
B252 | Bacillus subtilis isolated from tap water | [71] |
Plasmids | Â | Â |
pMAD | E. coli/B. licheniformis shuttle plasmid. ApR, EmR, ori Bacillus ts and pclpB-bgaB | [75] |
pMAD_SpR | pMAD-derivate supplemented with a SpR cassette in the SalI site. ApR, EmR, SpR, ori Bacillus ts and pclpB-bgaB | [76] |
pMAD_SpRΔgerAA | pMAD_SpR-derivate allowing substitution of parts of gerAA in MW3 with a SpR cassette. ApR, EmR, SpR, ori Bacillus ts and pclpB-bgaB | This study |
pHT315 | E. coli/B. licheniformis shuttle plasmid. ApR and EmR | [52] |
pHT315_MW3gerA | pHT315-derivate containing gerA fragmentbamplified from MW3 DNA template. ApR and EmR | This study |