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Table 1 Strains and plasmids used in this study

From: Role of the gerA operon in L-alanine germination of Bacillus licheniformisspores

strain or plasmid

description, phenotype or genotype relevant for this studya

reference

Strains

  

Escherichia coli TOP10

One Shot® TOP10 electro/chemically competent E. coli for cloning

Invitrogen

MW3

Bacillus licheniformis DSM13 (ΔhsdR1, ΔhsdR2)

[50]

NVH-1307

B. licheniformis MW3ΔgerAA::spc. SpR.

This study

NVH-1311

NVH-1307 with pHT315_MW3gerA. SpR and EmR.

This study

ATCC 14579

Bacillus cereus type strain

[72, 73]

B252

Bacillus subtilis isolated from tap water

[71]

Plasmids

  

pMAD

E. coli/B. licheniformis shuttle plasmid. ApR, EmR, ori Bacillus ts and pclpB-bgaB

[75]

pMAD_SpR

pMAD-derivate supplemented with a SpR cassette in the SalI site. ApR, EmR, SpR, ori Bacillus ts and pclpB-bgaB

[76]

pMAD_SpRΔgerAA

pMAD_SpR-derivate allowing substitution of parts of gerAA in MW3 with a SpR cassette. ApR, EmR, SpR, ori Bacillus ts and pclpB-bgaB

This study

pHT315

E. coli/B. licheniformis shuttle plasmid. ApR and EmR

[52]

pHT315_MW3gerA

pHT315-derivate containing gerA fragmentbamplified from MW3 DNA template. ApR and EmR

This study

  1. a ApR; resistance to ampicillin, EmR; resistance to erythromycin, SpR; resistance to spectinomycin, ori Bacillus ts; temperature-sensitive Bacillus origin of replication, pclpB-bgaB; constitutively expressed termostable β-galactosidase (allowing blue/white screening of transformants on X-Gal plates).
  2. b gerA fragment contains a sequence 151 bp upstream of gerAA, gerAA, gerAB, gerAC and 177 bp downstream of gerAC.