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Table 5 Comparison of virulence gene expression in the wild type and the gpsX mutant cultured in XVM2 medium by QRT-PCR a

From: The gpsX gene encoding a glycosyltransferase is important for polysaccharide production and required for full virulence in Xanthomonas citri subsp. citri

Gene locus_tag Function of protein product ΔΔC T ± SDb Fold change ± SD c P d
gumB XAC2585 EPS xanthan biosynthesis 0.2665 ± 0.1912 0.8314 ± 0.1102 0.0524
rfbC XAC3598 LPS O-antigen biosynthesis -0.2018 ± 0.1467 0.8695 ± 0.0841 0.0621
katE XAC1211 Monofunctional catalase 0.0758 ± 0.1346 0.9485 ± 0.0871 0.4407
pthA NS e TTSS effector -0.1703 ± 0.2407 1.1253 ± 0.1845 0.3128
hrpX XAC1266 TTSS regulator 0.2578 ± 0.1638 0.8364 ± 0.0997 0.2442
hrcV XAC0405 TTSS component 0.1828 ± 0.1348 0.8811 ± 0.0832 0.1119
  1. a Both 16S rRNA and gyrA genes were used as endogenous controls in the QRT-PCR experiments and similar results were obtained when the data were normalized against the two genes respectively. Only the data obtained with 16S rRNA gene as control were shown.
  2. b The mean ΔΔC T was determined using four biological repeats. The experiment was repeated two times with similar results. Data from one experiment are shown.
  3. c The expression change (mutant/wild type) in mutant 223 G4 (gpsX-) was calculated using 2-ΔΔCT.
  4. dP value, analyzed by Student's t -test. Values are significantly different when P is < 0.05.
  5. e No specific locus_tag. This represents the gene expression of pthA1, pthA2, pthA3 and pthA4.