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Table 2 Bacterial strains and plasmidsa

From: The gpsX gene encoding a glycosyltransferase is important for polysaccharide production and required for full virulence in Xanthomonas citri subsp. citri

Strains and plasmids Characteristics Reference or source
Strains   
E. coliDH5α F- recA1 endA1 hsdR17 supE44 thi-1 gyrA96 relA1 Δ (argE-lacZYA)169 φ80 lazA Δ M15 [29]
HB101 F- supE44, hsdS20(rB- mB-), recA13, ara-14, proA2, lacY1, galK2, rpsL20, xyl-5, mtl-1, leuB6, thi [30]
X. citri subsp. citri   
306 Syn. X. axonopodis pv. citri strain 306; wild type, Rfr [31]
223G4 (gpsX-) gpsX (XAC3110):: EZ-Tn5 derivative of strain 306, Kmr, Rfr [24]
223G4V (gpsX-) 223G4 (gpsX-) containing pUFR053, Cmr, Gmr, Kmr, Rfr This study
C223G4 (gpsX+) 223G4 (gpsX-) containing pJU3110, Cmr, Gmr, Kmr, Rfr This study
Plasmids   
pRK2013 ColE1 Kmr Tra+, conjugation helper plasmid [32]
pUFR053 IncW Mob+mob(P) lacZ+ Par+, Cmr, Gmr, shuttle vector [33]
pJU3110 2,299-bp KpnI- HindIII fragment containing wild-type gpsX cloned in pUFR053; Cmr, Gmr This study
  1. aApr, Cmr, Gmr, Kmr, and Rfr indicate resistance to ampicillin, chloromycetin, gentamicin, kanamycin and rifamycin, respectively.