Pyrosequencing data processing procedure | Processing algorithms | ||||
---|---|---|---|---|---|
 | PHRED-filteringa | Sequence length cut-off | Denoising | Filtering by SW mapping scoreb | Restriction of sequencesc |
1) Standard dT-RFLPd | >20e | >300 bp | Yes | >150f | Yes |
2) Filtered dT-RFLPe | >20 | >300 bp | No | >150 | Yes |
3) Raw dT-RFLPd | >20 | >300 bp | No | No (0)g | Yes |