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Table 1 RAPD Primers used for VIDISCR and the result of Virus discovery by the VIDISCR method

From: Identification of a novel Getah virus by Virus-Discovery-cDNA random amplified polymorphic DNA (RAPD)

Primer

Sequence (5’-3’)

SV5

SV40

YN08

S1

GTTTCGCTCC

N

N*

2/3

S2

TGATCCCTGG

N

1/3*

N

S3

CATCCCCCTG

2/2

N

N

S4

GGACTGGAGT

1/3

N

N

S5

TGCGCCCTTC

N

1/2

N

S11

GTAGACCCGT

1/3

N

1/1

S12

CCTTGACGCA

2/3

1/1

1/2

S13

TTCCCCCGCT

N

N

1/2

S14

TCCGCTCTGG

1/1

1/2

N

S15

GGAGGGTGTT

2/3

N

2/2

S21

CAGGCCCTTC

N

N

2/2

S22

TGCCGAGCTG

N

N

1/2

S23

AGTCAGCCAC

1/3

N

1/2

S24

AATCGGGCTG

N

N

N

S25

AGGGGTCTTG

N

0/2

1/2

S36

AGCCAGCGAA

2/4

N

N

S37

GACCGCTTGT

1/1

N

N

S38

AGGTGACCGT

N

N

0/1

S39

CAAACGTCGG

N

1/2

N

S40

GTTGCGATCC

N

N

1/2

  1. *Note: “N” denote The unique and prominent DNA fragments were not present in the test sample ; the denominator was the number of The unique and prominent DNA fragments by cloned and the numerator was the number of the virus DNA fragments in the test sample.