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Table 1 Characterization of the low-virulence L. monocytogenes strains

From: Polyphasic characterization and genetic relatedness of low-virulence and virulent Listeria monocytogenes isolates

Strains

Sub-cutaneous test

Phenotypic Groupc

Mutations

Genotypic Groupd

MLST

PFGE types

Mean (log spleens) ± S.D.a

I/Tb

Sequence types

ApaI

AscI

CHU 860776 e

0

0/5

I

PrfA K220T, truncated InlA (76 AA)

Ia

13

2

2

CNL 895803 e

0

0/5

I

PrfA K220T, truncated InlA (76 AA)

Ia

13

2

2

CNL 895804 e

0

0/5

I

PrfA K220T, truncated InlA (76 AA)

Ia

13

2

2

CNL 895806 e

0

0/5

I

PrfA K220T, truncated InlA (76 AA)

Ia

13

2

2

CNL 895809 e

0

0/5

I

PrfA K220T, truncated InlA (76 AA)

Ia

13

2a

2

CNL 895793 e

0

0/5

I

PrfA K220T, truncated InlA (76 AA)

Ia

13

2a

2

SO49 e

0

0/5

I

PrfA K220T, truncated InlA (76 AA)

Ia

13

3

23

AF10 e

0

0/5

I

PrfA K220T, truncated InlA (76 AA)

Ia

13

3

23

99EB24LM

0

0/5

I

PrfA K220T, truncated InlA (76 AA)

Ia

13

3

23

99EB04LM

0

0/5

I

PrfA K220T, truncated InlA (76 AA)

Ia

13

3

23

BO18 e

1.31

1/5

I

PrfAΔ174-237, truncated InlA (188 AA)

Ib

31

77a

61b

BO38 e

0

0/5

I

PrfAΔ174-237, truncated InlA (188 AA)

Ib

31

77a

61b

AF95 e

0

0/5

I

PrfAΔ174-237, truncated InlA (188 AA)

Ib

31

77a

61c

99EB15LM

0

0/5

I

PrfAΔ174-237, truncated InlA (188 AA)

Ib

31

21a

20

NP 26

0

0/5

I

PrfA K130Q

Ic

2

61a

3

454 e

3.26 ± 0.53

3/20

II

mutated PC-PLC (D61E, L183F, Q126K, A223V)

 

10

9

11

CNL 895807 e

3

1/25

III

truncated InlA (25 AA), mutated InlB (A117T, V132I), PI-PLC T262A

IIIa

193

1

1

416 e

0

0/5

III

truncated InlA (25 AA), mutated InlB (A117T, V132I), PI-PLC T262A

IIIa

193

1

1

417 e

2.81 ± 1.47

2/20

III

truncated InlA (25 AA), mutated InlB (A117T, V132I), PI-PLC T262A

IIIa

193

1

1

BO43 e

2.53

1/5

III

truncated InlA (25 AA), mutated InlB (A117T, V132I), PI-PLC T262A

IIIa

193

1a

1a

CNL 895795 e

0

0/5

III

truncated InlA (25 AA), mutated InlB (A117T, V132I), PI-PLC T262A

IIIa

193

1a

1a

DSS794AA1

0

0/5

III

truncated InlA (25 AA), mutated InlB (A117T, V132I), PI-PLC T262A

IIIa

193

144

33a

DSS1130BFA2

0.47

1/5

III

truncated InlA (25 AA), mutated InlB (A117T, V132I), PI-PLC T262A

IIIa

193

143

129

DPF234HG2

2.76 ± 0.04

2/5

III

truncated InlA (25 AA), mutated InlB (A117T, V132I), PI-PLC T262A

IIIa

193

145

33b

AF105 e

0

0/5

III

truncated InlA (576 AA)

IIIb

9

81

64

442 e

0

0/5

IV

  

1

6

7

02-99 SLQ 10c Al

2.9 ± 0.05

2/5

IV

  

1

11

7

3876

3.42 ± 0.2

3/5

IV

  

1

142

113

3877

2.7 ± 0.2

3/5

IV

  

1

142

113

N2

3.59 ± 0.48

2/5

IV

  

10

11

4b

CR282 e

3.01 ± 0.61

2/10

IV

  

195

158

85

LSEA 99–23 f

4.49 ± 0.89

3/5

IV

truncated InlA (576 AA)

 

9

21a

20

LSEA 99-4f

3.67 ± 0.81

3/5

IV

  

198

48

101

09-98 SRV 10a Al1

0

0/5

IV

  

4

37

38b

449 e

0

0/5

V

3 AA deletion at position 742 in InlA

 

194

8

6

BO34 e

3.63 ± 0.56

5/10

V

  

2

4a

3

464 e

2.59 ± 0.39

9/15

V

  

1

9c

4a

09-98 SRV 10b Al2

3.54 ± 0.27

3/5

V

  

54

135

124

11-99 SRV 1a Al

0

0/5

V

  

4

37

38b

09-98 HPR 50a Al1

0

0/5

V

3 AA deletion at position 742 in InlA

 

6

67a

98a

436 e

2.81 ± 0.68

12/20

VI

  

2

4

3

LSEA 00–14 f

0

0/5

VI

  

2

106

3a

04-99 EBS 1 lb Al

2.53 ± 1.76

2/5

VI

  

54

139

125

  1. a Log numbers of Listeria recovered from spleens three days after sub-cutaneous injection into the left hind footpads of immunocompetent Swiss mice with 104 CFU in 50 μL. Values are from infected mice.
  2. b Ratio of infected mice to inoculated mice in sub-cutaneous test.
  3. c These groups are based on combined values of cell invasion, plaque formation, and phospholipase activities.
  4. d These groups are based on sequencing of prfA, plcA, plcB, inlA and inlB genes.
  5. e Results already published in Roche et al. [7].
  6. f Results already published in Kerouanton et al. [10].