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Table 2 Combined dataset of full list of proteins identified in pool B after direct trypsin digestion and SDS-PAGE separation

From: Application of protein purification methods for the enrichment of a cytotoxin from Campylobacter jejuni

Accession number

Full identification name

Biological function known or inferred

Localisation

Mascot score

Total matches

Peptides matched

Sequence coverage (%)

A1VXN0 (Cj0146c)*

Thioredoxin reductase

Oxidation-reduction process

Cytoplasm

353

10

4

20

A1VXN1 (cj0147c)

Thioredoxin

Metabolic

Cytoplasm

1931

41

4

56

A1VXS1 (Cj0193c)

Trigger factor

Cell division

Cytoplasm

33

1

1

2

    

62c

4c

3c

6c

A1VXZ3 (Cj0269)

Branched-chain amino acid aminotransferase

Metabolic

Cytoplasm

146

10

3

17

A1VY51 (Cj0332c)

Nucleoside diphosphate kinase

Nucleotide metabolism

Cytoplasm

80

2

1

16

A1VY53 (Cj0334)

Antioxidant, AhpC/Tsa family

Cell redox homeostasis

Cytoplasm

1762

62

6

41

    

90c

5c

5c

33c

A1VYF1 (Cj0434)

2,3-bisphosphoglycerate-independent phosphoglycerate

Gluconeogenesis

Cytoplasm

70

6

3

6

    

50c

4c

4c

9c

A1VYI6 (Cj0470)

Elongation factor Tu (tuf)

Protein biosynthesis

Cytoplasm

94

7

3

10

    

53c

4c

3c

10c

A1VYJ5 (Cj0479)

DNA-directed RNA polymerase subunit beta

Transcription

Cytoplasm

48

5

1

0

A1VYM1 (Cj0509c)

ATP-dependent chaperone protein ClpB

Metabolic

Cytoplasm

110

6

2

3

    

100c

6c

5c

5c

A1VZ00 (Cj0640c)

Aspartyl-tRNA synthetase (aspS)

Protein biosynthesis

Cytoplasm

44

3

2

6

A1VZH9 (Cj0835c)

Aconitate hydratase

Metabolic

Cytoplasm

97

3

2

3

    

127c

7c

6c

11c

A1VZR2 (Cj0929)

Cytosol aminopeptidase family protein

Proteolysis

Cytoplasm

68

3

1

2

    

39c

3c

3c

5c

A1W057 (Cj1070)

30S ribosomal protein S6

Translation

Cytoplasm

226

8

1

8

A1W0G5 (Cj1181c)

Elongation factor Ts (tsf)

Protein biosynthesis

Cytoplasm

201

5

2

10

A1W0K3 (Cj1220)

10 kDa chaperonin (groS)

Protein folding

Cytoplasm

689

37

4

47

A1W0K4 (Cj1221)

60 kDa chaperonin (groL)

Protein folding

Cytoplasm

505

27

7

19

    

179c

6c

5c

13c

A8FKV4 (Cj0531)

Isocitrate dehydrogenase, NADP-dependent

Metabolic

Cytoplasm

38

7

3

6

    

355c

14c

12c

17c

A8FNK5 (Cj1545c)

MdaB-like protein

Oxidation-reduction process

Cytoplasm

81

3

2

12

A1VZC7 (Cj0779)

Thiol peroxidase

Oxidation-reduction process

Cytoplasm / periplasm

679

14

4

37

A8FKM7 (Cj0440c)

Putative transcriptional regulator

Transcription

Cytoplasma

82

3

3

12

A1VXI8 (Cj0105)

ATP synthase subunit alpha

ATP synthesis

Cytoplasmic membrane

84

3

2

9

A1W0Q1 (Cj1266c)

Quinone-reactive Ni/Fe-hydrogenase, large subunit (hydB)

Oxidation-reduction process

Cytoplasmic membrane

200

7

3

9

A1W0V5 (cj1338c)

Flagellin B

Flagellar motility

Extracellular

2023

48

8

23

    

608c

25c

13c

34c

A1VY12 (cj0289c)

Major antigenic peptide PEB3

Transport

Non-cytoplasmic

173

4

2

11

    

304c

8c

6c

27c

A1VZC6 (cj0778)

Major antigenic peptide PEB2

Transport

Non-cytoplasmic

233

9

3

27

    

159c

8c

6c

30c

A1VZK9 (Cj0866)

Arylsulfate sulfotransferase, degenerate

Transferase

Non-cytoplasmic

358

10

4

8

A1VZY6 (cj0998c)

Putative periplasmic protein

Unknown

Non-cytoplasmic

597

19

3

25

A1W0Q2(cj1240c)

Putative periplasmic protein

Protein binding

Non-cytoplasmic

151

11

3

13

A8FKY2 (Cj0559)

Putative pyridine nucleotide-disulphide oxidoreductase

Oxidation-reduction process

Non-cytoplasmic

200

7

3

9

    

292c

17c

13c

49c

A8FLP3 (cj0834c)

Putative uncharacterised protein

Protein-protein interaction

Non-cytoplasmic a

155c

8c

7c

19c

A8FKK7 (Cj0420)

Putative uncharacterised protein

Lipid binding protein

Non-cytoplasmic, likely periplasmic

331

12

2

14

A1VYD4 (Cj0414)

Putative oxidoreductase subunit

Oxidation-reduction process

Non-cytoplasmicb

581

11

3

26

A1VXJ7 (cj0114)

Putative periplasmic protein

Protein binding

Outer membrane

174

5

1

4

A1VXA8 (Cj0029)*

L-asparaginase

Metabolic

Periplasm

139

3

1

4

    

63c

3c

3c

14c

A1VXB4

Gamma-glutamyltransferase

Transferase

Periplasm

575

18

6

14

    

303c

23c

11c

19c

A1VXC2 (Cj0037c)

Putative cytochrome C

Electron carrier activity

Periplasm

46

1

1

5

A1VXM7 (Cj0143c)

Putative periplasmic solute binding protein for ABC transporter system

Metal ion binding

Periplasm

36

1

1

4

A1VY79 (Cj0358)

Putative cytochrome C551 peroxidase

Peroxidase

Periplasm

627

33

3

13

    

283c

22c

13c

38c

A1W0L1 (Cj1228c)

Serine protease

Proteolysis

Periplasm

239

11

8

31

A8FKK2 (Cj0415)

Putative GMC oxidoreductase subunit

Unknown

Unknown

1830

71

16

38

    

286c

15c

13c

28c

  1. *Gene designation refers to the best match identified in Campylobacter jejuni NCTC 11168 (where present).
  2. a The programme signal was used for protein localisation prediction; non-cytoplasmic localisation refers to proteins whose localisations are unknown, but a cytoplasmic localisation is ruled out as a N-terminal signalling sequence is present.
  3. b The uniprot annotation was used for protein localisation prediction.
  4. c The identification data obtained from the gel based analysis.
  5. Prediction of protein localisation was determined using the program PSORTb, and table listings are sorted by localisation.