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Table 2 Combined dataset of full list of proteins identified in pool B after direct trypsin digestion and SDS-PAGE separation

From: Application of protein purification methods for the enrichment of a cytotoxin from Campylobacter jejuni

Accession number Full identification name Biological function known or inferred Localisation Mascot score Total matches Peptides matched Sequence coverage (%)
A1VXN0 (Cj0146c)* Thioredoxin reductase Oxidation-reduction process Cytoplasm 353 10 4 20
A1VXN1 (cj0147c) Thioredoxin Metabolic Cytoplasm 1931 41 4 56
A1VXS1 (Cj0193c) Trigger factor Cell division Cytoplasm 33 1 1 2
     62c 4c 3c 6c
A1VXZ3 (Cj0269) Branched-chain amino acid aminotransferase Metabolic Cytoplasm 146 10 3 17
A1VY51 (Cj0332c) Nucleoside diphosphate kinase Nucleotide metabolism Cytoplasm 80 2 1 16
A1VY53 (Cj0334) Antioxidant, AhpC/Tsa family Cell redox homeostasis Cytoplasm 1762 62 6 41
     90c 5c 5c 33c
A1VYF1 (Cj0434) 2,3-bisphosphoglycerate-independent phosphoglycerate Gluconeogenesis Cytoplasm 70 6 3 6
     50c 4c 4c 9c
A1VYI6 (Cj0470) Elongation factor Tu (tuf) Protein biosynthesis Cytoplasm 94 7 3 10
     53c 4c 3c 10c
A1VYJ5 (Cj0479) DNA-directed RNA polymerase subunit beta Transcription Cytoplasm 48 5 1 0
A1VYM1 (Cj0509c) ATP-dependent chaperone protein ClpB Metabolic Cytoplasm 110 6 2 3
     100c 6c 5c 5c
A1VZ00 (Cj0640c) Aspartyl-tRNA synthetase (aspS) Protein biosynthesis Cytoplasm 44 3 2 6
A1VZH9 (Cj0835c) Aconitate hydratase Metabolic Cytoplasm 97 3 2 3
     127c 7c 6c 11c
A1VZR2 (Cj0929) Cytosol aminopeptidase family protein Proteolysis Cytoplasm 68 3 1 2
     39c 3c 3c 5c
A1W057 (Cj1070) 30S ribosomal protein S6 Translation Cytoplasm 226 8 1 8
A1W0G5 (Cj1181c) Elongation factor Ts (tsf) Protein biosynthesis Cytoplasm 201 5 2 10
A1W0K3 (Cj1220) 10 kDa chaperonin (groS) Protein folding Cytoplasm 689 37 4 47
A1W0K4 (Cj1221) 60 kDa chaperonin (groL) Protein folding Cytoplasm 505 27 7 19
     179c 6c 5c 13c
A8FKV4 (Cj0531) Isocitrate dehydrogenase, NADP-dependent Metabolic Cytoplasm 38 7 3 6
     355c 14c 12c 17c
A8FNK5 (Cj1545c) MdaB-like protein Oxidation-reduction process Cytoplasm 81 3 2 12
A1VZC7 (Cj0779) Thiol peroxidase Oxidation-reduction process Cytoplasm / periplasm 679 14 4 37
A8FKM7 (Cj0440c) Putative transcriptional regulator Transcription Cytoplasma 82 3 3 12
A1VXI8 (Cj0105) ATP synthase subunit alpha ATP synthesis Cytoplasmic membrane 84 3 2 9
A1W0Q1 (Cj1266c) Quinone-reactive Ni/Fe-hydrogenase, large subunit (hydB) Oxidation-reduction process Cytoplasmic membrane 200 7 3 9
A1W0V5 (cj1338c) Flagellin B Flagellar motility Extracellular 2023 48 8 23
     608c 25c 13c 34c
A1VY12 (cj0289c) Major antigenic peptide PEB3 Transport Non-cytoplasmic 173 4 2 11
     304c 8c 6c 27c
A1VZC6 (cj0778) Major antigenic peptide PEB2 Transport Non-cytoplasmic 233 9 3 27
     159c 8c 6c 30c
A1VZK9 (Cj0866) Arylsulfate sulfotransferase, degenerate Transferase Non-cytoplasmic 358 10 4 8
A1VZY6 (cj0998c) Putative periplasmic protein Unknown Non-cytoplasmic 597 19 3 25
A1W0Q2(cj1240c) Putative periplasmic protein Protein binding Non-cytoplasmic 151 11 3 13
A8FKY2 (Cj0559) Putative pyridine nucleotide-disulphide oxidoreductase Oxidation-reduction process Non-cytoplasmic 200 7 3 9
     292c 17c 13c 49c
A8FLP3 (cj0834c) Putative uncharacterised protein Protein-protein interaction Non-cytoplasmic a 155c 8c 7c 19c
A8FKK7 (Cj0420) Putative uncharacterised protein Lipid binding protein Non-cytoplasmic, likely periplasmic 331 12 2 14
A1VYD4 (Cj0414) Putative oxidoreductase subunit Oxidation-reduction process Non-cytoplasmicb 581 11 3 26
A1VXJ7 (cj0114) Putative periplasmic protein Protein binding Outer membrane 174 5 1 4
A1VXA8 (Cj0029)* L-asparaginase Metabolic Periplasm 139 3 1 4
     63c 3c 3c 14c
A1VXB4 Gamma-glutamyltransferase Transferase Periplasm 575 18 6 14
     303c 23c 11c 19c
A1VXC2 (Cj0037c) Putative cytochrome C Electron carrier activity Periplasm 46 1 1 5
A1VXM7 (Cj0143c) Putative periplasmic solute binding protein for ABC transporter system Metal ion binding Periplasm 36 1 1 4
A1VY79 (Cj0358) Putative cytochrome C551 peroxidase Peroxidase Periplasm 627 33 3 13
     283c 22c 13c 38c
A1W0L1 (Cj1228c) Serine protease Proteolysis Periplasm 239 11 8 31
A8FKK2 (Cj0415) Putative GMC oxidoreductase subunit Unknown Unknown 1830 71 16 38
     286c 15c 13c 28c
  1. *Gene designation refers to the best match identified in Campylobacter jejuni NCTC 11168 (where present).
  2. a The programme signal was used for protein localisation prediction; non-cytoplasmic localisation refers to proteins whose localisations are unknown, but a cytoplasmic localisation is ruled out as a N-terminal signalling sequence is present.
  3. b The uniprot annotation was used for protein localisation prediction.
  4. c The identification data obtained from the gel based analysis.
  5. Prediction of protein localisation was determined using the program PSORTb, and table listings are sorted by localisation.