Worms fed respiratory deficient E. coli have decreased coliform counts during early to mid adulthood. N2 worms were fed OP50, AN180, GD1 or AN120 as hatchlings and five worms were collected and mechanically disrupted at the designated age of adulthood. The lysate was analyzed for colony forming units as described in Experimental Procedures. Colony forming units (cfu/worm) were determined the following day. (Note that N2 L4 larvae contained on average less than 1 cfu/worm). Black diamonds, OP50; red squares, GD1; green triangles, AN180; blue circles, AN120. Asterisks indicate p-value < 0.05 when compared with the OP50 diet on the designated day. Data were subjected to one-way ANOVA with Fisher’s test at a significance level of p < 0.05 for each time point indicated.