Table 1 Bacterial strains and plasmids used in this work
Strain/plasmid | Relevant characteristics | Source/reference |
|---|---|---|
Bacterial strains | ||
NZ9000 | A derivative of L. lactis MG1363 wild type strain generated by the integration of the NisRK genes | 45 |
LL | L. lactis MG1363 containing pOri23 plasmid | 40 |
LL-mInlA+ | L. lactis NZ9000 strain containing pOri253:mInlA | This work |
LL-BLG | L. lactis MG1363 strain containing pOri23 and pValac: BLG plasmid | 32 |
LLmInlA+BLG | L. lactis NZ9000 strain expressing mInlA gene and carrying pValac: BLG plasmid | This work |
Plasmids | ||
pPL2:mInlA | E. coli vector containing mInlA gene | 30 |
pOri253Link | L. lactis-E. coli shuttle vector, Eryr | This work |
pOri23 | L. lactis-E. coli shuttle vector, Eryr | 40 |
pValac: BLG | L. lactis-E. coli shuttle vector carrying the BLG gene under the control of the eukaryotic promoter IE CMV, Cmr | 32 |
pOri253:mInlA | L. lactis-E. coli shuttle vector carrying the mInlA gene under the control of the constitutive PrfA promoter protein and harboring the native cell wall anchoring signal | This work |