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Table 2 Y. pestis strains and plasmids used in this study

From: Yersinia pestis insecticidal-like toxin complex (Tc) family proteins: characterization of expression, subcellular localization, and potential role in infection of the flea vector

Strain or Plasmid

Descriptiona

Source or reference

Strains

 KIM6+

Tc genes present, pCD1-negative

[33]

 KIM6+ΔyitR

Deletion of the regulator YitR

[9]

 KIM6+ΔyitA-yipB

Deletion of the Tc locus from YitA to YipB, YitR still present

This study

 KIM6+ (pCR-XL-TOPO::yitR)

KIM6+ transformed with a high-copy plasmid producing YitR (Kan, Zeo)

This study

 KIM6+ΔyitA-yipB (pCR-XL-TOPO::yitR)

KIM6+ΔyitA-yipB transformed with a high-copy plasmid producing YitR (Kan, Zeo)

This study

 KIM6+ (pWKS130::yitR)

KIM6+ transformed with a low-copy plasmid producing YitR (Kan)

This study

 KIM6+ΔyitA-yipB (pWKS130::yitR)

KIM6+ΔyitA-yipB transformed with a low-copy plasmid producing YitR (Kan)

This study

 KIM6+ YitA-β-lactamase

YitA::β-lactamase translational fusion

This study

 KIM6+ YipA-β-lactamase

YipA::β-lactamase translational fusion

This study

 KIM6+ YitA-β-lactamase (pCR-XL-TOPO::yitR)

KIM6+ YitA-β-lactamase transformed with a high-copy plasmid producing YitR (Kan, Zeo)

This study

 KIM6+ YipA-β-lactamase (pCR-XL-TOPO::yitR)

KIM6+ YipA-β-lactamase transformed with a high-copy plasmid producing YitR (Kan, Zeo)

This study

 KIM6+ (pCR-XL-TOPO::yitR) (pAcGFP1)

KIM6+ (pCR-XL-TOPO::yitR) transformed with a plasmid producing GFP (Kan, Zeo, Amp)

This study

 KIM6+ΔyitA-yipB (pCR-XL-TOPO::yitR) (pAcGFP1)

KIM6+ΔyitA-yipB (pCR-XL-TOPO::yitR) transformed with a plasmid producing GFP (Kan, Zeo, Amp)

This study

Plasmids

 pCR-XL-TOPO::yitR

yitR expressed under native promoter from a high-copy plasmid (Kan, Zeo)

This study

 pWKS130::yitR

yitR expressed under native promoter from a low-copy plasmid (Kan)

[9]

 pAcGFP1

Constitutively producing GFP (Amp)

Clontech

 pUC19

Cloning plasmid for creation of YitA- and YipA-β-lactamase fusion constructs (Amp)

[34]

 pDS132::yitA-β-lactamase

Suicide plasmid containing YitA-β-lactamase fusion construct (Cam)

This study

 pDS132::yipA-β-lactamase

Suicide plasmid containing YipA-β-lactamase fusion construct (Cam)

This study

 pET300/NT-DEST::yitA

Production of YitA with an N-terminal His tag (Amp)

This study

 pET300/NT-DEST::yipA

Production of YipA with an N-terminal His tag (Amp)

This study

  1. a antibiotic resistances where present are noted in parentheses: Kan = Kanamycin, Amp = Ampicillin, Cam = Chloramphenicol, Zeo = Zeocin.