Strain or Plasmid | Descriptiona | Source or reference |
---|---|---|
Strains | ||
 KIM6+ | Tc genes present, pCD1-negative | [33] |
 KIM6+ΔyitR | Deletion of the regulator YitR | [9] |
 KIM6+ΔyitA-yipB | Deletion of the Tc locus from YitA to YipB, YitR still present | This study |
 KIM6+ (pCR-XL-TOPO::yitR) | KIM6+ transformed with a high-copy plasmid producing YitR (Kan, Zeo) | This study |
 KIM6+ΔyitA-yipB (pCR-XL-TOPO::yitR) | KIM6+ΔyitA-yipB transformed with a high-copy plasmid producing YitR (Kan, Zeo) | This study |
 KIM6+ (pWKS130::yitR) | KIM6+ transformed with a low-copy plasmid producing YitR (Kan) | This study |
 KIM6+ΔyitA-yipB (pWKS130::yitR) | KIM6+ΔyitA-yipB transformed with a low-copy plasmid producing YitR (Kan) | This study |
 KIM6+ YitA-β-lactamase | YitA::β-lactamase translational fusion | This study |
 KIM6+ YipA-β-lactamase | YipA::β-lactamase translational fusion | This study |
 KIM6+ YitA-β-lactamase (pCR-XL-TOPO::yitR) | KIM6+ YitA-β-lactamase transformed with a high-copy plasmid producing YitR (Kan, Zeo) | This study |
 KIM6+ YipA-β-lactamase (pCR-XL-TOPO::yitR) | KIM6+ YipA-β-lactamase transformed with a high-copy plasmid producing YitR (Kan, Zeo) | This study |
 KIM6+ (pCR-XL-TOPO::yitR) (pAcGFP1) | KIM6+ (pCR-XL-TOPO::yitR) transformed with a plasmid producing GFP (Kan, Zeo, Amp) | This study |
 KIM6+ΔyitA-yipB (pCR-XL-TOPO::yitR) (pAcGFP1) | KIM6+ΔyitA-yipB (pCR-XL-TOPO::yitR) transformed with a plasmid producing GFP (Kan, Zeo, Amp) | This study |
Plasmids | ||
 pCR-XL-TOPO::yitR | yitR expressed under native promoter from a high-copy plasmid (Kan, Zeo) | This study |
 pWKS130::yitR | yitR expressed under native promoter from a low-copy plasmid (Kan) | [9] |
 pAcGFP1 | Constitutively producing GFP (Amp) | Clontech |
 pUC19 | Cloning plasmid for creation of YitA- and YipA-β-lactamase fusion constructs (Amp) | [34] |
 pDS132::yitA-β-lactamase | Suicide plasmid containing YitA-β-lactamase fusion construct (Cam) | This study |
 pDS132::yipA-β-lactamase | Suicide plasmid containing YipA-β-lactamase fusion construct (Cam) | This study |
 pET300/NT-DEST::yitA | Production of YitA with an N-terminal His tag (Amp) | This study |
 pET300/NT-DEST::yipA | Production of YipA with an N-terminal His tag (Amp) | This study |