Table 1 Oligonucleotides used in this study
From: Characterization of the Bat proteins in the oxidative stress response of Leptospira biflexa
Oligonucleotide | Sequence (5′ – 3′) | Function |
|---|---|---|
Lb.htpG.F | GTCTACATTGAGATGGATGTGG | Amplification of batABD + flanking sequences |
Lb.II0014.RC | CAGACCAATTACTCAAATGC | Amplification of batABD + flanking sequences |
batB.seq1.F | CAGCGATGGACTCTAGAAAATC | Amplification of batA + flanking sequences |
Lb.II0013/14.PCR1.RC | CTGTTGTTATCTTCGCTTCAC | Amplification of batA + flanking sequences |
batAKO.RC.NheI | agctagcGTTAGGTTATAAAATCCTTTTTG | Construction of allelic-exchange plasmids |
batKO.F.NheI | gctagcCATATGCAAGCTGAAGAAAAAGG | Construction of ΔbatABD allelic-exchange plasmid |
batAKO.F. NheI | gctagcATGGAAACAAATACGGTTATTTAC | Construction of ΔbatA allelic-exchange plasmid |
Pflg.NheI.F | gctagcTACCCGAGCTTCAAGGAAGATT | Amplification of kan |
Tkan.NheI.RC | gctagcGAGCTAGCGCCGTCCCGTCAA | Amplification of kan |
Lb.batA.F | CTGGGAACTGAGTTTCTTGG | Amplification of batA probe |
Lb.batA.RC | CTCGTCCTATCATCCTACAGG | Amplification of batA probe |
Lb.batB.RC | CCAGAACCAATCCAATGGGC | Amplification of batB/D probe |
batD.PCR1.RC | GAATTCGACTTCGACCGAG | Amplification of batB/D probe |
flaB.F.qPCR | CTGCTTACAGGAGCGTTTGCT | qPCR primer |
flaB.RC.qPCR | TGGTGCATGTTAGCTCCAATATG | qPCR primer |
flab.Lb.Probe | bACTCAACCCAACTGCTAGTATGTGGTT | qPCR probe |
batA.F.qPCR | AGGAGCCGCATACTTACAATCC | qPCR primer |
batA.RC.qPCR | GGATGTACCGGCTATCAGTTCAT | qPCR primer |
batA.probe | bCTTTCAAGTGACCGTTTTGCCT | qPCR probe |
batB.F.qPCR | CCTGGAACCGGGAAAGGT | qPCR primer |
batB.RC.qPCR | ATCACATTGTCGCCGTAAGGT | qPCR primer |
batB.probe | bCTTTGTTACTTACGATTCTAATTTGGTAG | qPCR probe |
batD.F.qPCR | TGTCGCTATGGTAGAAGGATTCG | qPCR primer |
batD.RC.qPCR | TGCGGACACTCCCTGTTTC | qPCR primer |
batD.probe | bAAAGAAATTACTTCCTCTCTGAGTTCTTAG | qPCR probe |
htpG.F.qPCR | TTTTCGGGAGCAACTGACTTC | qPCR primer |
htpG.RC.qPCR | TCCTAGTCCAAAATGGCCTATGAT | qPCR primer |
htpG.probe | bCCAAACAGTACCAGAACACAGAAAATAAGGCAG | qPCR probe |
phoR.F.qPCR | CGTTTGATTCGCAGGGTGAT | qPCR primer |
phoR.RC.qPCR | TTAGGCTCCAAGGCAGATAAAATT | qPCR primer |
phoR.probe | bAAGCGGTGCAAACTGCACTCAATTTTG | qPCR probe |