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Table 1 Strains and plasmids used in this study

From: Staphylococcus aureus autoinducer-2 quorum sensing decreases biofilm formation in an icaR-dependent manner

Strain or plasmid

Description

Reference or source

RN6390B

Standard laboratory strain

NARSAa

RN4220

8325-4 r-

NARSA

ΔluxS

RN6390B luxS::ermB

This study

RN6911

RN6390B derivative; agr locus replaced with tetM cassette

NARSA

ΔagrΔluxS

RN6911 luxS::ermB, agr/luxS double mutant

This study

ΔluxSpluxS

Complemented strain of ΔluxS; Apr Cmr

This study

RN6390BG

RN6390B/pgfp

This study

ΔluxSG

ΔluxS/pgfp

This study

RN6911G

RN6911/pgfp

This study

ΔagrΔluxSG

ΔagrΔluxS/pgfp

This study

NCTC8325

Standard Laboratory strain

NARSA

NCTC8325ΔluxS

NCTC8325 luxS::ermB

60

E. coli strains

  

TOP10

Cloning

Invitrogen

Plasmids

  

pEASY-Blunt

Clone vector, Kanr Apr

Transgen

pBTluxS

Vector used for luxS mutagenesis, Apr Cmr Emr

60

pLI50

E. coli-S. aureus shuttle cloning vector, Apr Cmr

Addgene

pLIluxS

pLI50 with luxS and its promoter, Apr Cmr

60

pgfp

gfp expression with the promoter of S10 ribosomal gene, Apr, Cmr

 
  1. aNARSA, Network on Antimicrobial Resistance in Staphylococcus aureus.