Figure 6

Additive effect played by the LuxS/AI-2 QS system and the agr -mediated QS system. (A) The ΔagrΔluxSG and RN6911G grew biofilms in the flow cell, and the representative images were measured by CLSM at the 3rd and 5th day of biofilm formation. Strains are indicated in the figure. (B) Overnight cultures of WT (RN6390B), Δagr (RN6911), ΔluxS and Δagr ΔluxS were inoculated in 24-well plate and formed biofilms under anaerobic conditions. (C) WT, Δagr, ΔluxS and Δagr ΔluxS formed 5 days biofilms in a flow cell on the upper surface of the coverslips, which were cut and examined by scanning electron microscopy. (D) The anaerobic jar was used for monitoring the biofilm formation of the WT, Δagr, ΔluxS and Δagr ΔluxS, OD₅₆₀ was measured after crystal violet staining.