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Table 3 Bacterial strains and plasmids used in this study

From: Quorum-sensing regulates biofilm formation in Vibrio scophthalmi

Strain or plasmid

Genotype and feature(s)

Reference

V. scophthalmi strains

  

A089

Wild type, turbot isolate (CECT 4638T)

[2]

A102

Wild type, turbot isolate (CECT 5965)

[1, 2]

A089_23

A089 ΔluxR mutant

This study

A089_88

A089_23 (pMMB207)

“

A089_75

A089_23 (pMMB207::luxR) mutant

“

A089_68

A089 ΔluxS mutant

“

A089_84

A089_68 (pMMB207::luxS) mutant

“

A089_92

A089_68 (pMMB207)

“

A102_56

A102 ΔluxR mutant

“

A102_78

A102_56 (pMMB207::luxR) mutant

“

A102_90

A102_56 (pMMB207)

“

A102_73

A102 ΔluxS mutant

“

A102_87

A102_73 (pMMB207::luxS) mutant

“

A102_94

A102_73 (pMMB207)

“

A102_pACYC

A102 (pACYC184)

[11]

A102_6.2

A102 (pACYC184::aiiA)

“

A102_99

A102_73 (pACYC::luxS)

This study

E. coli strains

  

DH5α

E. coli used for transformation: λpir

Promega

S17-1

E. coli used for conjugation: λpir mob

[32]

Plasmids

  

pDM4

Cmr Kanr SacBR; suicide vector

[28]

pMMB207

Cmr,Ptac, broad host range expression vector

[33]

pACYC184

Tetr, Cmr, broad host range expression vector

[34]

pGEM T-easy

Ampr Kanr; TA cloning vector for sequencing

Promega