From: Quorum-sensing regulates biofilm formation in Vibrio scophthalmi
Strain or plasmid | Genotype and feature(s) | Reference |
---|---|---|
V. scophthalmi strains | Â | Â |
A089 | Wild type, turbot isolate (CECT 4638T) | [2] |
A102 | Wild type, turbot isolate (CECT 5965) | |
A089_23 | A089 ΔluxR mutant | This study |
A089_88 | A089_23 (pMMB207) | “ |
A089_75 | A089_23 (pMMB207::luxR) mutant | “ |
A089_68 | A089 ΔluxS mutant | “ |
A089_84 | A089_68 (pMMB207::luxS) mutant | “ |
A089_92 | A089_68 (pMMB207) | “ |
A102_56 | A102 ΔluxR mutant | “ |
A102_78 | A102_56 (pMMB207::luxR) mutant | “ |
A102_90 | A102_56 (pMMB207) | “ |
A102_73 | A102 ΔluxS mutant | “ |
A102_87 | A102_73 (pMMB207::luxS) mutant | “ |
A102_94 | A102_73 (pMMB207) | “ |
A102_pACYC | A102 (pACYC184) | [11] |
A102_6.2 | A102 (pACYC184::aiiA) | “ |
A102_99 | A102_73 (pACYC::luxS) | This study |
E. coli strains | Â | Â |
DH5α | E. coli used for transformation: λpir | Promega |
S17-1 | E. coli used for conjugation: λpir mob | [32] |
Plasmids | Â | Â |
pDM4 | Cmr Kanr SacBR; suicide vector | [28] |
pMMB207 | Cmr,Ptac, broad host range expression vector | [33] |
pACYC184 | Tetr, Cmr, broad host range expression vector | [34] |
pGEM T-easy | Ampr Kanr; TA cloning vector for sequencing | Promega |