Real time quantitative PCR (qPCR) of RNA helicases from G. lamblia during antigenic variation. The relative expressions were calculated after induction of antigenic variation for 30 min – 1 hour (empty fill pattern) and for 3 to 4 hours (line fill pattern). The relative expression from different helicases was divided into up-regulated (upper panel) and down-regulated (lower panel). Green bars represent significant up-regulation and red bars represent significant down-regulation, gray bars represent no change in the relative expression. A. Helicases up-regulated during the first 30 min to 1 h. B. Helicases up-regulated at 3 to 4 h. C. Helicases down-regulated during the first 30 min to 1 h. D. Helicases down-regulated at 3 to 4 h. Center inset: relative expression for Giardia Dicer and Argonaute at earlier or later time points. The ORFs are indicated at the bottom of the graph. The graphs represent the mean of three different measures and the respective standard deviation.