Table 1 Sequences of primers
From: Correlation of intestinal microbiota with overweight and obesity in Kazakh school children
Primer Name | Sequence (5’-3’) | Tm (°C) | Target length |
|---|---|---|---|
Firm-primer-F | GTCAGCTCGTGTCGTGA | 60°C | 178 bp |
Firm-primer-R | CCATTGTAKYACGTGTGT | 60°C | |
Firm-probe | VIC-GTCAANTCATCATGCC-MGBNFQ | 65°C | |
Bact-primer-F | AGCAGCCGCGGTAAT | 60°C | 183 bp |
Bact-primer-R | CTAHGCATTTCACCGCTA | 60°C | |
Bact-probe | FAM-CCCTTTAAACCC-MGBNFQ | 65°C |