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Table 1 Sequences of primers

From: Correlation of intestinal microbiota with overweight and obesity in Kazakh school children

Primer Name Sequence (5’-3’) Tm (°C) Target length
Firm-primer-F GTCAGCTCGTGTCGTGA 60°C 178 bp
Firm-primer-R CCATTGTAKYACGTGTGT 60°C  
Firm-probe VIC-GTCAANTCATCATGCC-MGBNFQ 65°C  
Bact-primer-F AGCAGCCGCGGTAAT 60°C 183 bp
Bact-primer-R CTAHGCATTTCACCGCTA 60°C  
Bact-probe FAM-CCCTTTAAACCC-MGBNFQ 65°C