Lysis protein of phage M. (A) The lysis gene. The Shine-Dalgarno sequence is underlined and initiation and termination codons are indicated by green and pink shading, respectively. The translated amino acid sequence is given above the RNA sequence and the putative transmembrane helix is shaded gray. (B) An RNA hairpin around the initiation codon of the lysis gene. The initiation codon and the Shine-Dalgarno sequence are indicated. (C) Verification of the lysis gene. Growth of E.coli cells harboring either empty vector (pET28) or a plasmid with the cloned lysis gene (pET28-LP) before and after the induction of protein synthesis is shown.