Skip to main content

Table 2 qPCR analysis of paramagnetic bead captured bacteria from food samples a

From: Highly specific fiber optic immunosensor coupled with immunomagnetic separation for detection of low levels of Listeria monocytogenes and L. ivanovii

Paramagnetic bead (PMB) Detection/enumeration of PMB captured cells by qPCR (CFU/ml)
  Hotdogb Soft Cheeseb
  CFU/ml ± SD % CFU/ml ± SD %
MyOne-2D12 1.09 ± 3.07 × 107 12.62 ± 3.5A 2.65 ± 1.79 × 107 16.2 ± 9.7A
MyOne-3F8 2.26 ± 1.18 × 106 2.63 ± 1.4B 6.45 ± 7.44 × 106 3.8 ± 4.3B
Dynabead anti-Listeria 2.76 ± 3.11 × 106 6.12 ± 0.5B 7.65 ± 8.26 × 106 4.4 ± 4.8B
  1. aqPCR analysis is based on hlyA. Primers to 16S gene sequences were used as internal control.
  2. bData are average of 3 experiments run in triplicate. Values labeled with letters (A, B) in a column are significantly different at P < 0.05.