Highly specific fiber optic immunosensor coupled with immunomagnetic separation for detection of low levels of Listeria monocytogenes and L. ivanovii

Table 2 qPCR analysis of paramagnetic bead captured bacteria from food samples ^a

Paramagnetic bead (PMB)	Detection/enumeration of PMB captured cells by qPCR (CFU/ml)
	Hotdog^b		Soft Cheese^b
	CFU/ml ± SD	%	CFU/ml ± SD	%
MyOne-2D12	1.09 ± 3.07 × 10⁷	12.62 ± 3.5^A	2.65 ± 1.79 × 10⁷	16.2 ± 9.7^A
MyOne-3F8	2.26 ± 1.18 × 10⁶	2.63 ± 1.4^B	6.45 ± 7.44 × 10⁶	3.8 ± 4.3^B
Dynabead anti-Listeria	2.76 ± 3.11 × 10⁶	6.12 ± 0.5^B	7.65 ± 8.26 × 10⁶	4.4 ± 4.8^B

^aqPCR analysis is based on hlyA. Primers to 16S gene sequences were used as internal control.
^bData are average of 3 experiments run in triplicate. Values labeled with letters (A, B) in a column are significantly different at P < 0.05.

ISSN: 1471-2180