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Table 2 Biochemical properties of the three enzymes

From: Identification of the para-nitrophenol catabolic pathway, and characterization of three enzymes involved in the hydroquinone pathway, in pseudomonas sp. 1-7

Enzyme Temperature range(°C) Optimal temperature Thermal Stability pH range Optimal pH Acid stability Alkali Stability Specific activity
PdcDE 20-70 40°C 35% 3.0-10.0 6.0 20% 60% ND
PdcG 20-70 50°C 65% 5.0-10.0 8.0 18% 75% 0.44 U/mg
PdcF 20-70 40°C 10% 5.0-9.0 7.0 20% 58% 446.97 U/mg
  1. Relative activity of purified protein when it was treated in 60°C for 20 min;
  2. Relative activity of purified protein when it was treated in pH 3.0 for 30 min;
  3. Relative activity of purified protein when it was treated in pH 10.0 for 30 min;
  4. Not detectedEach value represents the mean of at least three independent replicates.