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Table 2 Biochemical properties of the three enzymes

From: Identification of the para-nitrophenol catabolic pathway, and characterization of three enzymes involved in the hydroquinone pathway, in pseudomonas sp. 1-7

Enzyme

Temperature range(°C)

Optimal temperature

Thermal Stability

pH range

Optimal pH

Acid stability

Alkali Stability

Specific activity

PdcDE

20-70

40°C

35%

3.0-10.0

6.0

20%

60%

ND

PdcG

20-70

50°C

65%

5.0-10.0

8.0

18%

75%

0.44 U/mg

PdcF

20-70

40°C

10%

5.0-9.0

7.0

20%

58%

446.97 U/mg

  1. Relative activity of purified protein when it was treated in 60°C for 20 min;
  2. Relative activity of purified protein when it was treated in pH 3.0 for 30 min;
  3. Relative activity of purified protein when it was treated in pH 10.0 for 30 min;
  4. Not detectedEach value represents the mean of at least three independent replicates.