Table 1 Bacterial strains and plasmids used in this study
Strains and plasmids | Relevant genotype or characteristic(s) | Reference or source |
|---|---|---|
Pseudomonas sp | ||
Strain 1-7 | methyl parathion and p-nitrophenol utilizer, wild type | This study |
E.coli | ||
Trans10 | F-Φ80(lacZ) M15 lacX74hsdR(rK-mK+) recA1398endA1tonA | TransGen |
BL21(DE3) | F- ompT hsdS (rB- mB-) gal dcm lacY1(DE3) | Novagen |
Plasmids | ||
pET30a | Kmr, Expression vector | Novagen |
pET22b | Ampr, Expression vector | Novagen |
pET2230 | Ampr, Expression vector | This study |
pEASY-T3 | Ampr, Cloning vector | TransGen |
pET30- pdcF | BamHI-HindIII fragment containing pdcF inserted into pET30a | This study |
pET30- pdcG | BamHI-XhoI fragment containing pdcG inserted into pET30a | This study |
pET30- pdcD | BamHI-XhoI fragment containing pdcD inserted into pET30a | This study |
pET2230- pdcE | BamHI-XhoI fragment containing pdcE inserted into pET2230 | This study |