Table 2 List of strains used in this work

DH5α

F' fhuA2 Δ(argF-lacZ)U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17a

[52]

CMA601

E. coli DH5α carrying pSDA-02

This work

BL21(DE3)

F^–ompT gal dcm lon hsdS_B(r_B^- m_B^-) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])

[53]

CMA602

E. coli BL21(DE3) overexpressing His-tagged RNase R from S. pneumoniae TIGR4

[54]

CMA603

E. coli BL21(DE3) carrying pSDA-02

This work

S. pneumoniae

JNR7/87 (TIGR4)

[51]

TIGR4 RNase R^-

TIGR4 rnr^- (Δrnr-Cm^R)

C. Arraiano and P. Lopez Labs^a

CMA604

TIGR4 rnr^- (Δrnr-Cm^R) carrying pIL253 (Ery^R) expressing RNase R

This work

CMA605

TIGR4 smpB^- (ΔsmpB-Kan^R)

This work

CMA606

TIGR4 smpB^- (ΔsmpB-Kan^R) carrying pLS1GFP (Ery^R) expressing SmpB

This work