Growth on and uptake of acetate of a deh4p–dehp2–double mutant. (A) Wildtype MBA4 (■) and deh4p– dehp2– double mutant (□) were grown in minimal medium containing acetate. Seed cultures were grown in LB– and sub-cultured into minimal medium containing acetate at 30°C. The optical densities of the cultures were determined at 600 nm (OD600) with a spectrophotometer. (B) Acetate-uptake rates of acetate-grown- wildtype and double mutant. Uptakes of 50 μM of [2-14C]acetate were assayed by a filtration method for a period of 2 min. Data shown are the means of three independent experiments, and the error bars represent the standard deviations.