Colocalization between GHSV-UL46 and Rab27a in the TGN. HOG cells cultured in DM and infected at a m.o.i. of 1 with GHSV-UL46 were fixed and processed for confocal triple-label indirect immunofluorescence analysis with anti-Rab27a and anti-TGN-46 polyclonal antibodies. Low panels, corresponding to confocal slices of 0.8 μm, are enlargements of the square shown in upper panel, which corresponds to the projection of the planes obtained by confocal microscopy. Images show colocalization between Rab27a and GHSV-UL46 in the TGN. Colocalization between Rab27a and GHSV-UL46 appears cyan; between Rab27a and TGN, magenta; between GHSV-UL46 and the TGN, yellow; colocalization between Rab27a, GHSV-UL46 and TGN appears white. (DIC: Differential Interference Contrast).