Inter- and Intra-subtype genotypic differences that differentiate Mycobacterium avium subspecies paratuberculosisstrains

Table 2 Discriminatory Index of IS 900 RFLP, MIRU-VNTR and SnaB1, Spe1 PFGE typing used alone and in combination

Typing methods		S type			C type¹	All types
	Subtypes	I	III	I + III	II
IS900 RFLP	No.²	10	14	24	35	59
IS900 RFLP	DI	0.356	0.934	0.873	0.644	0.856
PFGE SnaB1	No.	5	10	15	24	39
PFGE SnaB1	DI	1.000	0.956	0.990	0.895	0.960
PFGE Spe1	No.	5	10	15	24	39
PFGE Spe1	DI	1.000	0.978	0.990	0.801	0.924
PFGE (SnaB1-Spe1)	No.	5	10	15	24	39
PFGE (SnaB1-Spe1)	DI	1.000	1.000	1.000	1.000	1.000
MIRU-VNTR	No.	10	14	24	35	59
MIRU-VNTR	DI	0.644	0.89	0.801	0.876	0.925
IS900 RFLP + MIRU-VNTR	No.	10	14	24	35	59
	DI	0.644	0.736	0.935	0.965	0.977

¹: Panel of strains selected to represented the whole diversity of RFLP and MIRU-VNTR profiles of type C strains.
²: No. Number of strains analyzed.

ISSN: 1471-2180