Minimum spanning tree based on MIRU-VNTR genotypes among Mycobacterium avium subsp. paratuberculosis of types S and C, Mycobacterium avium subsp. avium, Mycobacterium avium subsp. hominissuis, and Mycobacterium avium subsp. silvaticum. 135 strains were isolated from cattle (sky blue), 23 strains from sheep (orange), 17 strains from goat (dark blue), 63 strains from pigs (light green), 17 strains from birds (yellow), 17 strains from humans (white), 6 strains from deer (purple), 5 strains from other sources (red), 4 strains from wood pigeons (brown), and 2 different vaccine strains (316 F from France and United Kingdom) (light blue). Each genotype is displayed as a pie chart, the size of which is proportional to the number of strains, with color-coded distribution of the strain origins. The number of loci differing between the genotypes is indicated by the style of the connecting lines: thick and short, 1 difference; intermediate, 2 differences; thin and long: 3 differences.