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Table 2 Bacteria and plasmids used in this study

From: Toxin-antitoxin loci vapBC-1 and vapXD contribute to survival and virulence in nontypeable Haemophilus influenzae

Strain Description Source
86-028NP NTHi nasopharyngeal isolate from a pediatric otitis media patient R. S. Munson, Jr.
BL21(DE3) fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS λ DE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5 Invitrogen
DH5α λ―Φ80ΔlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK―mK―)supE44 thi-1 gyrA relA1 Laboratory collection
SU202 lexA71::Tn5(Def)sulA211 Δ(IacIPOZYA)169/F’lacIqlacZM15::Tn9 M. Granger-Schnarr
Plasmid Description Source
pGEM5 Cloning vector Promega
pUC4K Source of the kanamycin cassette Lab collection
pBluescript II Cloning vector Lab collection
pUCΔEcat Source of the chloramphenicol cassette Lab collection
pET24b Expression vector Novagen
pSR658 LexA DBD fusion vector [31]
pSR659 LexA DBD fusion vector [31]
pDD689 pET24b with the cat gene [30]
pDD731 pGEM5 with the vapBC-1 gene deletion This work
pDD788 pBluescript II SK(+) with the vapXD deletion This work
pDD791 pET24b with vapXD This work
pDD859 pSR658 with vapC-1 fused to the LexA DBD This work
pDD866 pSR658 with vapB-1 fused to the LexA DBD This work
pDD867 pSR659 with vapB-1 fused to the LexA DBD This work
pDD868 pSR659 with vapC-1 fused to the LexA DBD This work
pDD882 pSR658 with vapX fused to the LexA DBD This work
pDD883 pSR659 with vapX fused to the LexA DBD This work
pDD884 pSR659 with vapD fused to the LexA DBD This work
pDD885 pSR658 with vapD fused to the LexA DBD This work
pDD902 pET24b with vapX This work