Table 2 Bacteria and plasmids used in this study
Strain | Description | Source |
|---|---|---|
86-028NP | NTHi nasopharyngeal isolate from a pediatric otitis media patient | R. S. Munson, Jr. |
BL21(DE3) | fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS λ DE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5 | Invitrogen |
DH5α | λ―Φ80ΔlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK―mK―)supE44 thi-1 gyrA relA1 | Laboratory collection |
SU202 | lexA71::Tn5(Def)sulA211 Δ(IacIPOZYA)169/F’lacIqlacZM15::Tn9 | M. Granger-Schnarr |
Plasmid | Description | Source |
pGEM5 | Cloning vector | Promega |
pUC4K | Source of the kanamycin cassette | Lab collection |
pBluescript II | Cloning vector | Lab collection |
pUCΔEcat | Source of the chloramphenicol cassette | Lab collection |
pET24b | Expression vector | Novagen |
pSR658 | LexA DBD fusion vector | [31] |
pSR659 | LexA DBD fusion vector | [31] |
pDD689 | pET24b with the cat gene | [30] |
pDD731 | pGEM5 with the vapBC-1 gene deletion | This work |
pDD788 | pBluescript II SK(+) with the vapXD deletion | This work |
pDD791 | pET24b with vapXD | This work |
pDD859 | pSR658 with vapC-1 fused to the LexA DBD | This work |
pDD866 | pSR658 with vapB-1 fused to the LexA DBD | This work |
pDD867 | pSR659 with vapB-1 fused to the LexA DBD | This work |
pDD868 | pSR659 with vapC-1 fused to the LexA DBD | This work |
pDD882 | pSR658 with vapX fused to the LexA DBD | This work |
pDD883 | pSR659 with vapX fused to the LexA DBD | This work |
pDD884 | pSR659 with vapD fused to the LexA DBD | This work |
pDD885 | pSR658 with vapD fused to the LexA DBD | This work |
pDD902 | pET24b with vapX | This work |