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Table 1 Strains and plasmids used in this study

From: PHB granules are attached to the nucleoid via PhaM in Ralstonia eutropha

Strain Relevant characteristic Source or reference
Escherichia coli JM109 Cloning strain  
E. coli S17-1 Conjugation strain [45]
Ralstonia eutropha H16 Wild type strain, PHB accumulation DSMZ 428
Ralstonia eutropha HF39 Streptomycin resistant derivate of H16 [22, 39]
R. eutropha H16 ∆phaP5 Chromosomal deletion of phaP5 [22]
R. eutropha H16 ∆phaM Chromosomal deletion of phaM [32]
Plasmid Relevant feature(s) Source or reference
pBBR1MCS-2 broad host range vector [46]
pBBR1MCS2- PphaC-eyfp-c1 Constitutive eYfp over-expression [22]
pBBR1MCS-2- P phaC -eyfp-phaP5 Fusion of PhaP5 to C-terminus of eYfp [22]
pBBR1MCS-2- P phaC –eyfp-phaM Fusion of eYfp to N-terminus to PhaM [32]
pBBR1MCS-2- P phaC –phaP5 Constitutive over-expression of PhaP5 this study
pBBR1MCS-2- P phaC –phaM Constitutive over-expression of PhaM this study