Table 1 Strains and plasmids used in this study

Escherichia coli JM109

Cloning strain

E. coli S17-1

Conjugation strain

[45]

Ralstonia eutropha H16

Wild type strain, PHB accumulation

DSMZ 428

Ralstonia eutropha HF39

Streptomycin resistant derivate of H16

[22, 39]

R. eutropha H16 ∆phaP5

Chromosomal deletion of phaP5

[22]

R. eutropha H16 ∆phaM

Chromosomal deletion of phaM

[32]

Plasmid

Relevant feature(s)

Source or reference

pBBR1MCS-2

broad host range vector

[46]

pBBR1MCS2- PphaC-eyfp-c1

Constitutive eYfp over-expression

[22]

pBBR1MCS-2- P _phaC -eyfp-phaP5

Fusion of PhaP5 to C-terminus of eYfp

[22]

pBBR1MCS-2- P _phaC –eyfp-phaM

Fusion of eYfp to N-terminus to PhaM

[32]

pBBR1MCS-2- P _phaC –phaP5

Constitutive over-expression of PhaP5

this study

pBBR1MCS-2- P _phaC –phaM

Constitutive over-expression of PhaM

this study