Time course of PHB granule formation in R. eutropha H16 ∆phaM. All preparations were performed as described in legend to Figure 1. Note, deletion of phaM resulted in formation of decreased number of big PHB granules. Incubation times in NB-gluconate medium for 0 min (a), 30 min (b), 60 min (c) and 180 min in (d). Bar 0.2 μm.