|
Primer
|
Sequence (5’-3’)
|
Reference
|
|---|
|
Forward
|
Reverse
|
|---|
|
cesD
|
GTTTATCAAATCATGAAGATGCACAA
|
GCCCTGGGATCTTGCATAAC
|
[23]
|
|
escJ
|
CCAATGATGTCAATGTTTCCAAA
|
GCGCGAACAAAATCCTCTTT
|
[23]
|
|
escR
|
GCCAGCCTCCAACAAGAATG
|
ATTGGCCTTGGGTATGATGATG
|
[23]
|
|
escU
|
TCCACTTTGTATCTCGGAATGAAG
|
CAAGGATACTGATGGTAACCCTGAA
|
[23]
|
|
flhC
|
CGCTTTCCAGCATCTGCAA
|
CGGGATATTCAGCTGGCAAT
|
[23]
|
|
flhD
|
TCATTCAGCAAGCGTGTTGAG
|
TCCCGCGTTGACGATCTC
|
[23]
|
|
ler
|
CGACCAGGTCTGCCCTTCT
|
TCGCTCGCCGGAACTC
|
[23]
|
|
sepZ
|
CGGAGACGAGCAGCACAGA
|
CCGCCAACCGCAGTAAGA
|
[23]
|
|
stx2
|
ACCCCACCGGGCAGTT
|
GTCAAAACGCGCCTGATAGAC
|
[23]
|
|
rpoA
|
GTTGCCGCACGACGAATCGC
|
CCCAATCGGCCGTCTGCTGG
|
This study
|
|
qseC
|
CAGTCCACAGGGCAGCGTGG
|
AGTCCACTGCCGGTAGCGGT
|
This study
|
|
qseB
|
GAGCTGCGCCACGGTAACGT
|
AGTTTGCGCGGCAGTACCCG
|
This study
|
|
qseA
|
CCAGCCCCCGACCTGATTGC
|
GCGGGATCAGGCGAGTCGAG
|
This study
|
|
qseB
(cloning)
|
GTGCTGTACAGAGCTC GTTACAAC
|
CCAGGCGACAAAGCTT GAAAGCA
|
This study
|
|
qseC
(cloning)
|
TGCGTCTGGGAGCTC ACGATTATC
|
GGTGAGACGTTTGTCGAC TATAGTACG
|
This study
|
- The underlined segment in AV25/26 and AV29/30 indicate the restriction enzyme sites.
