Table 1 Bacterial Strains used in the study
Strain/Plasmid | Genotype | Reference/Source |
|---|---|---|
Strains | Â | Â |
E. coli O157:H7 EDL933 | Wild type | ATCC (#43895) |
TEVS232 | E. coli TE2680 LEE1:lacZ | [41] |
TEVS21 | E. coli TE2680 LEE2:lacZ | [41] |
VS145 | EHEC 86–24 ΔqseA | [42] |
VS151 | VS145 with plasmid pVS150 | [42] |
VS138 | EHEC 86–24 ΔqseC | [6] |
VS179 | VS138 with plasmid pVS178 | [6] |
AV43 | WT with plasmid pVS178 | This study |
AV45 | WT with pVS150 | This study |
AV46 | TEVS232 with pVS150 | This study |
AV48 | WT with pAV11 | This study |
AV49 | WT with pAV12 | This study |
Plasmids | Â | Â |
pVS150 | qseA into pACYC177 | [42] |
pVS178 | E. coli K12 qseBC in pBAD33 | [6] |
pAV11 | EHEC qseC in pBAD33 | This Study |
pAV12 | EHEC qseB in pBAD33 | This study |
pBAD33 | pBAD33 | ATCC |