Effect of limonoids on EHEC adhesion and survival of Caco-2 cells. (A) Adhesion of EHEC to Caco-2 cells. Caco-2 cells were infected with 50 fold EHEC ATCC 43895 for 3 h. The EHEC cell numbers were enumerated by lysing the Caco-2 cells and plating the lysate on LB-agar plates, followed by counting colonies after 24 h. The data represents mean of three biological replicates and SD. Asterisk denotes significant (p<0.05) difference from solvent control (DMSO). (B) Survival of Caco-2 cells in presence of 100 μg/ml limonoids. The cell viability was measured by LDH assay after 6 h of growth in presence of limonoids.