Figure 2

Three parameter models of biofilm formation inhibition by citrus limonoids. Line curves at 50% and 25% represent the IC₅₀ and IC₂₅ values for compounds. Biofilms were grown in 96-well plates and quantified using crystal violet. Percent inhibition over solvent control (DMSO) was calculated. To generate 3-parameter models, concentrations were changed to Log10 μM and plotted against percent inhibition.