Respiratory proteins contribute differentially to Campylobacter jejuni’s survival and in vitro interaction with hosts’ intestinal cells

Table 1 Summary of the phenotypes associated with the RPs mutants

Mutant	Motility (Micro)		Res. H₂O₂ (Micro)		Res. H₂O₂ (Ana)		Biofilm (Micro)		Biofilm (Ana)		Biofilm (O₂)		PIC (42°C)		INT-407 (37°C)			Cell shape
Mutant	37°C	42°C	37°C	42°C	37°C	42°C	37°C	42°C	37°C	42°C	37°C	42°C	Adh	Inv	Adh	Inv	Intra	37°C	42°C
Δ *napA*	↑	↑	↓	↓	↓	↓	NS	NS	↑	NS	↓	↓	NS	NS	↓	NS	NS	Normal
Δ *nrfA*	↑	↑	NS	NS	NS	NS	NS	NS	↑	NS	NS	NS	↑	NS	NS	NS	↑	Normal
Δ *mfrA*	↑	↑	↑	↑	↑	↑	↓	↑	NS	↓	NS	NS	NS	NS	↓	↓	NS	Normal
Δ *hydB*	NS	NS	NS	NS	NS	NS	NS	NS	NS	NS	NS	NS	↓	↓	↓	↓	↓	Filament
Δ *fdhA*	↓	↓	↓	↓	↓	↓	↓	NS	NS	NS	NS	NS	↓	↓	NS	↓	↓	Bulging

Res. H₂O₂ and PIC indicate resistance to hydrogen peroxide and primary chicken intestinal epithelial cells, respectively. Microaerobic, anaerobic, and ambient oxygen incubation conditions are abbreviated as “Micro”, “Ana” and “O₂” respectively, while adherence, invasion and intracellular survival are abbreviated as “Adh”, “Inv” and “Intra”. Statistically significant increases or decreases (P < 0.05) as compared to the wildtype are indicated by ↑ and ↓, respectively, while NS indicates no significant differences.

ISSN: 1471-2180