From: Dual role of HupF in the biosynthesis of [NiFe] hydrogenase in Rhizobium leguminosarum
Primer | Sequence (5′-3′) | Use |
---|---|---|
HUPF5 | GCGGCACTGCTGGTCGGCTGAATCACTCCCAAGGCTGAGGTGTAGGCTGGAGCTGCTTC | hupF deletion |
HUPF3 | CGGCGGCAATTCCGGCTCGCGGGAAATGAGATCGGCGAACATATGAATATCCTCCTTAGT | |
HUPK52 | ACATTCCTTCTCGGGGCCGGCACGATAGGGATCGACGTGATTCCGGGGATCCGTCGACC | hupK deletion |
HUPK32 | TGCGATAGGCTGCGAGCCTGCCGTCACCGCCGATTTCGAGTGTAGGCTGGAGCTGCTTC | |
HYPC5 | GAACGAACATGCATCAATCGAGGAGAACCGGACATGTGCATTCCGGGGATCCGTCGACC | hypC deletion |
HYPC3 | TCGTCGATATATTTCATGCCGCATCTCCCATCGCACGTTGTGTAGGCTGGAGCTGCTTC | |
TAGF31 | TCCCGCGAGCCGGAATTGCCGCCGCATTTGCGCGGCGAGTGGAGCCACCCGCAGTTCGA | Generation of hupF ST fusion (pALPF382) |
TAGF32 | GGTGGAAACTCAAATTCCATTTTGGAAGTTCTCTTTTCAATATGAATATCCTCCTTAGT | |
FNDE | CTGTCAGTCGTCATATGTGCATCGGCATTCCCAT | Generation of pPM501/pPM501C plasmids |
MANG3 | ACGGCGGCGGGAATGCTC | |
HUPF-3413 L-Strep | GAGTACTCTCAGGCGCCTTTTTCGAACTGCGGGTGGCTCCAGCTAGCTCCCTCCACCGCTTCGGCAAGTCCGGCGAG | Generation of pPM501C plasmid |