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Table 4 Oligonucleotides used in this work

From: Dual role of HupF in the biosynthesis of [NiFe] hydrogenase in Rhizobium leguminosarum

Primer

Sequence (5-3)

Use

HUPF5

GCGGCACTGCTGGTCGGCTGAATCACTCCCAAGGCTGAGGTGTAGGCTGGAGCTGCTTC

hupF deletion

HUPF3

CGGCGGCAATTCCGGCTCGCGGGAAATGAGATCGGCGAACATATGAATATCCTCCTTAGT

HUPK52

ACATTCCTTCTCGGGGCCGGCACGATAGGGATCGACGTGATTCCGGGGATCCGTCGACC

hupK deletion

HUPK32

TGCGATAGGCTGCGAGCCTGCCGTCACCGCCGATTTCGAGTGTAGGCTGGAGCTGCTTC

HYPC5

GAACGAACATGCATCAATCGAGGAGAACCGGACATGTGCATTCCGGGGATCCGTCGACC

hypC deletion

HYPC3

TCGTCGATATATTTCATGCCGCATCTCCCATCGCACGTTGTGTAGGCTGGAGCTGCTTC

TAGF31

TCCCGCGAGCCGGAATTGCCGCCGCATTTGCGCGGCGAGTGGAGCCACCCGCAGTTCGA

Generation of hupF ST fusion (pALPF382)

TAGF32

GGTGGAAACTCAAATTCCATTTTGGAAGTTCTCTTTTCAATATGAATATCCTCCTTAGT

FNDE

CTGTCAGTCGTCATATGTGCATCGGCATTCCCAT

Generation of pPM501/pPM501C plasmids

MANG3

ACGGCGGCGGGAATGCTC

HUPF-3413  L-Strep

GAGTACTCTCAGGCGCCTTTTTCGAACTGCGGGTGGCTCCAGCTAGCTCCCTCCACCGCTTCGGCAAGTCCGGCGAG

Generation of pPM501C plasmid