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Table 2 Hydrogenase activity induced by R. leguminosarum strains in microaerobic cultures and in pea bacteroids

From: Dual role of HupF in the biosynthesis of [NiFe] hydrogenase in Rhizobium leguminosarum

Strain Genotype Hydrogenase activitya
   1% O2 3% O2 Bacteroids
UPM1155(pALPF1) wild type 13440 ± 1720 4970 ± 472 5980 ± 852
UPM1155 (pALPF14) ΔhypC < 200 < 200 < 200
UPM1155 (pALPF5) ΔhupF < 200 < 200 470 ± 84
UPM1155 (pALPF5/pPM501) ΔhupF/hupFST 12890 ± 230 5900 ± 779 5350 ± 728
UPM1155(pALPF5/pPM501C) ΔhupF/hupFCST 4780 ± 228 1025 ± 255 5400 ± 683
  1. aValues, expressed in nmoles H2 h-1 (mg protein)-1, are the average of three independent assays ± SE.